CAJ | 학술논문

采用茶多酚和壳聚糖对中国对虾进行冷藏保鲜处理,以虾肉蛋白质组成、肌动球蛋白盐溶性、巯基含量、Ca 2+-ATPase活性及SDS-PAGE图谱为指标,分析了两种保鲜剂对中国对虾肌肉蛋白特性的影响.结果显示,与对照组相比,保鲜处理组肌动球蛋白质的盐溶性、巯基含量和Ca 2+-ATPase活性下降速度减慢,SDS-PAGE分析亦可发现蛋白质降解速度有一定程度的降低,说明茶多酚和壳聚糖能够在一定程度上减缓蛋白质的变性.壳聚糖和茶多酚两种保鲜剂处理之间相比,发现壳聚糖对蛋白质的保护作用优茶多酚,这与前期得到的壳聚糖用中国对虾保鲜效果优茶多酚的研究结果一致.
채용다다분화각취당대중국대하진행랭장보선처리,이하육단백질조성、기동구단백염용성、구기함량、Ca 2+-ATPase활성급SDS-PAGE도보위지표,분석료량충보선제대중국대하기육단백특성적영향.결과현시,여대조조상비,보선처리조기동구단백질적염용성、구기함량화Ca 2+-ATPase활성하강속도감만,SDS-PAGE분석역가발현단백질강해속도유일정정도적강저,설명다다분화각취당능구재일정정도상감완단백질적변성.각취당화다다분량충보선제처리지간상비,발현각취당대단백질적보호작용우다다분,저여전기득도적각취당용중국대하보선효과우다다분적연구결과일치.
Chinese Shrimp (F.chinensis) were treated with tea polyphenols and chitosan respectively .The Effects of those two bio -preservatives on biochemical characteristics of the muscle proteins were evaluated by analyzing protein composition, salt solubilities, sulfhydryl contents, Ca 2+-ATPase activities and SDS-PAGE maps.The results showed that tea polyphenols and chitosan could slow down the decrease of the salt solubilities , total contents of sulfhydryl and Ca 2 +-ATPase activities of actomyosin significantly.The SDS -PAGE analysis showed that the both treatments can delay the degradation of muscle proteins of shrimp during chilled storage . Moreover, chitosan exhibited greater protective effect on protein denaturation than tea polyphenols , which agreed well with the previous result that chitosan showed better preservation effects than tea polyphenols on Chinese Shrimp during chilled storage.