CAJ | 학술논문

应用长爪沙鼠建立日本血吸虫病动物模型,观察实验性日本血吸虫病肝脏肉芽肿的超微结构改变.采用腹部贴片法感染血吸虫尾蚴,每只沙鼠感染(120±2)条尾蚴,于5周后解剖,取出肝脏做病理组织学和透射电镜观察.结果发现,肝脏切片 H.E.(铁苏木素伊红)染色后可观察到虫卵肉芽肿产生,透射电镜观察,可见超微结构改变.肝脏肉芽肿的超微结构的病变可以清晰地显示肉芽肿的发生机制,为实验性研究血吸虫病的防治奠定了基础.
응용장조사서건립일본혈흡충병동물모형,관찰실험성일본혈흡충병간장육아종적초미결구개변.채용복부첩편법감염혈흡충미유,매지사서감염(120±2)조미유,우5주후해부,취출간장주병리조직학화투사전경관찰.결과발현,간장절편 H.E.(철소목소이홍)염색후가관찰도충란육아종산생,투사전경관찰,가견초미결구개변.간장육아종적초미결구적병변가이청석지현시육아종적발생궤제,위실험성연구혈흡충병적방치전정료기출.
To observe the ultrastructural changes of hepatic egg granuloma of experimental Schistosomiasis japonicum, the gerbil mice model of Schistosomiasis is used. The mice have been infected individually with cercariae of Schistosoma japonicum (120±2), all of which were autopsied at the fifth week after infection. The liver was sectioned and observed by HE staining and electronic transmissive microscope. Results show that the nodules on the surface of liver and egg granuloma have been observed, and intact egg and ultrastructural changes have been noted. This research establishes the gerbil model of Schistosomiasis, and the liver egg granuloma is investigated. The results are expected to add some helpful references for the prevention of Schistosomiasis.