CAJ | 학술논문

目的建立测定海豹油软胶囊中有效成分二十碳五烯酸(EPA)、二十二碳六烯酸(DHA)、二十二碳五烯酸(DPA)含量的方法.方法以氰基柱色谱柱为分析柱,乙腈-水(90∶10)为流动相,检测波长220 nm.结果 EPA甲酯、DHA甲酯和DPA甲酯线性范围分别为0.6~3.6μg,0.6~3.6μg,0.3~1.8μg,加样回收率分别为99.75%,100.37%,100.35%,RSD分别为1.19%,0.87%,1.22%.结论本方法简便、快速、稳定、重复性好,可用于海豹油软胶囊的质量控制.
목적건립측정해표유연효낭중유효성분이십탄오희산(EPA)、이십이탄륙희산(DHA)、이십이탄오희산(DPA)함량적방법.방법이청기주색보주위분석주,을정-수(90∶10)위류동상,검측파장220 nm.결과 EPA갑지、DHA갑지화DPA갑지선성범위분별위0.6~3.6μg,0.6~3.6μg,0.3~1.8μg,가양회수솔분별위99.75%,100.37%,100.35%,RSD분별위1.19%,0.87%,1.22%.결론본방법간편、쾌속、은정、중복성호,가용우해표유연효낭적질량공제.
@@@@Objective To establish determination method of three active constituents in seal oil soft capsule, including eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and docosapentaenoic acid (DPA). Methods Cyano column is used as analytical column and acetonitrile-water (90∶10) is used as mobile phase. The determination wavelength is 220 nm. Results The linearity range of EPA methyl ester, the DHA methyl ester and the DPA methyl ester specimen is 0.6-3.6 μg, 0.6-3.6 μg and 0.3-1.8 μg, respectively. The average recovery is 99.75%, 100.37% and 100.35%, respectively. RSD is 1.19%, 0.87% and 1.22%, respectively. Conclusion This method is simple, fast, stable and of good reproducibility, thus can be used in quality control of the seal oil soft capsule.