CAJ | 학술논문

从桑天牛排泄物中分离产纤维素酶的菌株并进行鉴定,对其酶学特性以及饲料粗纤维的降解效果进行研究.采用刚果红染色法筛选出1株产纤维素酶的菌株HY3,利用形态学和分子生物学进行种属鉴定,并通过DNS法测定所产纤维素酶活力,用尼龙袋法测定其对三种饲料(麸皮、麦草秸秆、燕麦秸秆)粗纤维的降解率.结果表明,菌株HY3为地衣芽孢杆菌,此菌株所产纤维素酶的最适反应温度为70℃,最适反应pH值为6.5、菌株HY3对麸皮的粗纤维降解率最高(7.41%),对麦草秸秆粗纤维的降解率最差(4.89%).结果提示,该菌株所产纤维素酶具有较好的热稳定性,不耐强酸碱;粗纤维的降解率随时间的变化不大.
종상천우배설물중분리산섬유소매적균주병진행감정,대기매학특성이급사료조섬유적강해효과진행연구.채용강과홍염색법사선출1주산섬유소매적균주HY3,이용형태학화분자생물학진행충속감정,병통과DNS법측정소산섬유소매활력,용니룡대법측정기대삼충사료(부피、맥초갈간、연맥갈간)조섬유적강해솔.결과표명,균주HY3위지의아포간균,차균주소산섬유소매적최괄반응온도위70℃,최괄반응pH치위6.5、균주HY3대부피적조섬유강해솔최고(7.41%),대맥초갈간조섬유적강해솔최차(4.89%).결과제시,해균주소산섬유소매구유교호적열은정성,불내강산감;조섬유적강해솔수시간적변화불대.
Screening and identifying the strain producing cellulase from the excretion of Mulber?ry longicorn and its enzymatic properties and degradation rate of the crude fiber in the feed were studied as well. A cellulase-producing bacterial strain named HY3 was screened by Con?go red staining method, identified by morphologic and biologic observation. Its enzyme activities and degradation rates of the crude fiber in three kinds of feed(bran, wheat straw, oat straw) were examined by DNS method and nylon bag method, respectively. The results showed that the strain was identified as Bacillus licheniformis, the optimum temperature of the reaction with sub?strate was 70 ℃, the optimum pH was 6.5, the degradation rate to crude fiber of bran was the highest(7.41%), and the degradation rate to wheat straw was the lowest(4.89%). These results in?dicated that the cellulase it produced had good heat stability and weak acid-base tolerance, the degradation rate of crude fiber had no significant change over time.