CAJ | 학술논문

外源杀虫基因能否在转基因棉花中高效、稳定表达是影响转基因棉花应用前景的关键.本文从转基因棉花杀虫蛋白表达水平出发,通过在转基因棉花品种的种子中混杂非转基因棉花品种的种子,研究不同品种纯度的转基因棉花杀虫蛋白表达水平对转基因抗虫棉的抗虫性影响.本研究选用2个转基因抗虫棉品种GK12和SGK321,设置了8种混杂比例,通过分子检测和室内生测试验表明,随着抗虫纯度的下降,棉铃虫危害等级升高,幼虫校正死亡率下降,品种抗虫能力下降;从田间实验结果来看,随着抗虫纯度的下降,铃数减少,空果枝数增多,最终致使产量大幅下降,从而出现所谓的“抗虫棉不抗虫”现象.结果显示,转基因抗虫棉品种抗性纯度至少要达到97%,才可以保证产量不至于损失太大.
외원살충기인능부재전기인면화중고효、은정표체시영향전기인면화응용전경적관건.본문종전기인면화살충단백표체수평출발,통과재전기인면화품충적충자중혼잡비전기인면화품충적충자,연구불동품충순도적전기인면화살충단백표체수평대전기인항충면적항충성영향.본연구선용2개전기인항충면품충GK12화SGK321,설치료8충혼잡비례,통과분자검측화실내생측시험표명,수착항충순도적하강,면령충위해등급승고,유충교정사망솔하강,품충항충능력하강;종전간실험결과래간,수착항충순도적하강,령수감소,공과지수증다,최종치사산량대폭하강,종이출현소위적“항충면불항충”현상.결과현시,전기인항충면품충항성순도지소요체도97%,재가이보증산량불지우손실태대.
@@@@The efficient and stable expression of exogenous insecticidal genes in transgenic cotton is the key of transgenic cotton application. In order to compare the insect-resistant effect of transgenic cotton under different seeds purity,two transgenic varieties GK12 and SGK321 were mixed with non-genetically modified cotton seeds in eight different proportion for evaluating insecticidal protein expression level,the molecular detection and indoor hygiene tests showed that with the decline in the purity of insect resistance,cotton bollworm damage level gradually increased,corrected mortality of H. armigera larvae decreased, varieties of insect-resistant capacity declined. The field experiments indicated that with the decline of the insect resistant purity, boll number reduced,the number of empty fruit branches increased,which ultimately led to a sharp decline in production,and thus gave rise to the so-called “Bt cotton is not insect resistant”. In conclusion,the decline of seed purity had significant effection on insect resistance and cotton yield. In order to ensure high yield,the purity of transgenic insect-resistant cotton should be higher than 97% .