CAJ | 학술논문

目的:初步探究十五肽 BPC-157调节人脐静脉内皮细胞(HUVEC)功能的信号通路作用机制.方法:首先利用生物芯片筛选 BPC-157参与激活的细胞信号转导通路途径,进而通过 real-time PCR 证实 BPC-157对候选信号通路中相关基的 mRNA 表达水平的影响,最后采用 Western 印迹观察 BPC-157对候选信号通路中相关蛋白的磷酸化水平影响.结果:10μg/mL BPC-157作用 HUVEC 24 h 后,信号转导通路发现者芯片结果显示,与18条信号转导通路相关的96个关键基中分别有4个基的 mRNA 表达水平上调和下调,其中与 MAPK 信号通路相关的3个关键基 c-Fos、c-Jun 和 Egr-1的 mRNA 表达水平显著性上调;低剂量 BPC-157(1μg/mL)作用 HUVEC 12 h 后,能够促进早期即刻基 c-Fos、c-Jun 和 Egr-1的 mRNA 表达水平;10μg/mL BPC-157作用 HUVEC 30 min 后,可明显促进 ERK1/2、p38蛋白磷酸化.结论:BPC-157可能通过活化 MAPK 信号转导通路途径后,激活下游早期即刻基转录,启动靶基的表达,从而发挥促进 HUVEC 增殖、迁移等功能.
목적:초보탐구십오태 BPC-157조절인제정맥내피세포(HUVEC)공능적신호통로작용궤제.방법:수선이용생물심편사선 BPC-157삼여격활적세포신호전도통로도경,진이통과 real-time PCR 증실 BPC-157대후선신호통로중상관기적 mRNA 표체수평적영향,최후채용 Western 인적관찰 BPC-157대후선신호통로중상관단백적린산화수평영향.결과:10μg/mL BPC-157작용 HUVEC 24 h 후,신호전도통로발현자심편결과현시,여18조신호전도통로상관적96개관건기중분별유4개기적 mRNA 표체수평상조화하조,기중여 MAPK 신호통로상관적3개관건기 c-Fos、c-Jun 화 Egr-1적 mRNA 표체수평현저성상조;저제량 BPC-157(1μg/mL)작용 HUVEC 12 h 후,능구촉진조기즉각기 c-Fos、c-Jun 화 Egr-1적 mRNA 표체수평;10μg/mL BPC-157작용 HUVEC 30 min 후,가명현촉진 ERK1/2、p38단백린산화.결론:BPC-157가능통과활화 MAPK 신호전도통로도경후,격활하유조기즉각기전록,계동파기적표체,종이발휘촉진 HUVEC 증식、천이등공능.
Objective: We probe into the pentadecapeptide BPC-157 induced function alterations of human umbili?cal vein endothelial cells(HUVEC) and the underlying mechanism. Methods: First, we screened the BPC-157 re?lated signal pathways by microarray; then, real-time PCR had been executed to affirm that BCP-157 could regu?late several signal molecules expression on mRNA level which involve in the collected signal pathways; at last, the phosphorylation level of the pathways was detected by Western-blot. Results: After 10 μg/mL BPC-157 treat?ed HUVEC for 24 h, microarray which includes 96 key genes belonged to 18 different pathways showed that 4 genes level had been regulated. c-Fos, c-Jun and Egr-1, three important molecules pertain to MAPK pathway, were significantly up regulated. BPC-157 also could enhance the expression of the three immediate early genes even in a low dosage(1 μg/mL). The phosphorylated ERK1/2 and p38 all enriched in HUVEC treated by 10 μg/mL BPC-157 for 30 min. Conclusion: BPC-157 promotes the proliferation and migration of human umbilical vein endothelial cells may attribute to its activated stimulation of MAPK signal pathway and immediate early genes ex?pression.