CAJ | 학술논문

目的:对北京地区成人呼吸道感染患者中人冠状病毒(HCoV)HKU1的感染状况进行初步分析,了解其流行分布、临床特点,并确定流行株的基型别.方法:2011年1月~2012年1月从北京地区成人呼吸道感染患者中收集鼻咽拭子标本559份,利用靶向棘突蛋白 S 与核心蛋白 N 的2种巢式 PCR 方法进行 HCoV-HKU1筛查,并对阳性片段进行序列测定,以确定其基型别;同时,对阳性标本进行常见呼吸道病毒的共感染筛查,进而分析 HCoV-HKU1感染的临床表现和流行病学特点.结果:559份鼻咽拭子标本中检出 HCoV-HKU1阳性9例(1.61%),其中3例合并HCoV-OC43感染;感染患者临床表现为急性呼吸道症状,以发热(88.9%)、咽痛(66.7%)、寒颤(68%)、流涕(55.6%)和咳嗽(44.4%)为主,其中1例有系统性红斑狼疮史;阳性基片段系统进化分析发现这9例感染均为 HCoV-HKU1 B型.结论:北京地区近年成人呼吸道感染患者中 HCoV-HKU1感染率较低(1.61%),其流行株基型为 B 型.
목적:대북경지구성인호흡도감염환자중인관상병독(HCoV)HKU1적감염상황진행초보분석,료해기류행분포、림상특점,병학정류행주적기형별.방법:2011년1월~2012년1월종북경지구성인호흡도감염환자중수집비인식자표본559빈,이용파향극돌단백 S 여핵심단백 N 적2충소식 PCR 방법진행 HCoV-HKU1사사,병대양성편단진행서렬측정,이학정기기형별;동시,대양성표본진행상견호흡도병독적공감염사사,진이분석 HCoV-HKU1감염적림상표현화류행병학특점.결과:559빈비인식자표본중검출 HCoV-HKU1양성9례(1.61%),기중3례합병HCoV-OC43감염;감염환자림상표현위급성호흡도증상,이발열(88.9%)、인통(66.7%)、한전(68%)、류체(55.6%)화해수(44.4%)위주,기중1례유계통성홍반랑창사;양성기편단계통진화분석발현저9례감염균위 HCoV-HKU1 B형.결론:북경지구근년성인호흡도감염환자중 HCoV-HKU1감염솔교저(1.61%),기류행주기형위 B 형.
Objective: To study the molecular epidemiological and clinical profiles of human coronavirus(HCoV) HKU1 among adults with acute respiratory infection(ARI) in Beijing. Methods: The nest RT-PCR assay targeting to spike(S) and nucleocapsid(N)coding region of HCoV-HKU1 was developed and used for screening HCoV-HKU1 infection among 559 nasopharyngeal swab specimens, which collected from adults with ARI in Beijing be?tween January 2011 and January 2012. The demographic and clinical data associated with HCoV-HKU1 infection were examined. Co-infection with other HCoV or respiratory viruses was also investigated by RT-PCR or multiple RT-PCR. The genotype distribution of HCoV-HKU1 infection was identified by sequence analysis of PCR positive products. Results: 9 of 559(1.61%) specimens were detected as positive for HCoV-HKU1 by nest RT-PCR, 3 of them were found co-infected with HCoV-OC43. The clinical profiles were indicated as fever(88.9%), pharyngitis (66.7%), rigors(68%), nasal discharge(55.6%) and cough(44.4%), and one of 9 patients with SLE. Sequence analysis showed that all of the HCoV-HKU1 infections fell into genotype B. Conclusion: Our data showed that HCoV-HKU1 prevalence was low(1.61%) among adults with ARI in Beijing area, the genotype distribution of HCoV-HKU1 was identified as B.