生物技术通讯
生物技術通訊
생물기술통신
LETTERS IN BIOTECHNOLOGY
2013年
2期
219-221
,共3页
自然杀伤细胞%CpG-ODN%重组乙型肝炎表面抗原%IFN-γ
自然殺傷細胞%CpG-ODN%重組乙型肝炎錶麵抗原%IFN-γ
자연살상세포%CpG-ODN%중조을형간염표면항원%IFN-γ
natural killer cells%CpG-ODN%rHBsAg%IFN-γ
目的:研究重组乙型肝炎表面抗(HBsAg)佐剂 BW006对小鼠脾自然杀伤(NK)细胞表面分子 CD69的表达和γ干扰素(IFN-γ)分泌水平的影响.方法:BW006、HBsAg 单用或联用体外刺激小鼠脾 NK 细胞,流式细胞仪检测NK 细胞膜表面分子 CD69的表达水平,ELISA 检测 IFN-γ的分泌水平.结果:5μg BW006体外刺激小鼠脾 NK 细胞24 h 后,NK 细胞表面分子 CD69的表达达峰值(阳性率45.18%),显著高40μg HBsAg 组(21.44%)(P<0.05),与5μg BW006和40μg HBsAg 联用组(58.49%)相比无显著差异;24 h 时,5μg BW006组的 IFN-γ分泌水平达56.95 ng/mL,显著高40μg HBsAg 组(8.74 ng/mL)(P<0.05),与联用组(57.70 ng/mL)相比无显著差异.结论:BW006具有上调 NK 细胞表面分子 CD69表达和诱导 IFN-γ分泌的早期活化 NK 细胞的功能,作为疫苗的新型佐剂前景较好.
目的:研究重組乙型肝炎錶麵抗(HBsAg)佐劑 BW006對小鼠脾自然殺傷(NK)細胞錶麵分子 CD69的錶達和γ榦擾素(IFN-γ)分泌水平的影響.方法:BW006、HBsAg 單用或聯用體外刺激小鼠脾 NK 細胞,流式細胞儀檢測NK 細胞膜錶麵分子 CD69的錶達水平,ELISA 檢測 IFN-γ的分泌水平.結果:5μg BW006體外刺激小鼠脾 NK 細胞24 h 後,NK 細胞錶麵分子 CD69的錶達達峰值(暘性率45.18%),顯著高40μg HBsAg 組(21.44%)(P<0.05),與5μg BW006和40μg HBsAg 聯用組(58.49%)相比無顯著差異;24 h 時,5μg BW006組的 IFN-γ分泌水平達56.95 ng/mL,顯著高40μg HBsAg 組(8.74 ng/mL)(P<0.05),與聯用組(57.70 ng/mL)相比無顯著差異.結論:BW006具有上調 NK 細胞錶麵分子 CD69錶達和誘導 IFN-γ分泌的早期活化 NK 細胞的功能,作為疫苗的新型佐劑前景較好.
목적:연구중조을형간염표면항(HBsAg)좌제 BW006대소서비자연살상(NK)세포표면분자 CD69적표체화γ간우소(IFN-γ)분비수평적영향.방법:BW006、HBsAg 단용혹련용체외자격소서비 NK 세포,류식세포의검측NK 세포막표면분자 CD69적표체수평,ELISA 검측 IFN-γ적분비수평.결과:5μg BW006체외자격소서비 NK 세포24 h 후,NK 세포표면분자 CD69적표체체봉치(양성솔45.18%),현저고40μg HBsAg 조(21.44%)(P<0.05),여5μg BW006화40μg HBsAg 련용조(58.49%)상비무현저차이;24 h 시,5μg BW006조적 IFN-γ분비수평체56.95 ng/mL,현저고40μg HBsAg 조(8.74 ng/mL)(P<0.05),여련용조(57.70 ng/mL)상비무현저차이.결론:BW006구유상조 NK 세포표면분자 CD69표체화유도 IFN-γ분비적조기활화 NK 세포적공능,작위역묘적신형좌제전경교호.
Objective: To study the influences of CpG adjuvant BW006 for hepatitis B vaccine on the pheno?type and functions of murine spleen natural killer(NK) cells. Methods: After murine spleen NK cells were stimu?lated with BW006 and/or HBsAg in vitro, the expression of CD69 on NK cells surface was analyzed by flow cy?tometer and the secretion level of IFN-γ was detected by ELISA. Results: After 24 hours of stimulation, the ex?pression of CD69 reached peak and the expression rate was 48.18% which was significantly higher than 40 μg HBsAg group(21.44%, P<0.05). However, the expression of CD69 on NK cells surface have no significant differ?ence in 5 μg BW006 group and 5 μg BW006 combined with 40 μg HBsAg group(58.49%). The IFN-γ secre?tion levels of murine spleen NK cells was up to 56.95 ng/mL after activating with 5 μg BW006 which was signifi?cantly higher than 40 μg HBsAg group(8.47 ng/mL, P<0.05). However, the IFN-γ secretion levels of murine spleen NK cells have no significant difference in 5 μg BW006 group and 5 μg BW006 combined with 40 μg HB?sAg group(57.70%). Conclusion: BW006 functions to activate NK cells on early stage, regulate expression of CD69 and induce IFN-γ secretion, indicating BW006 to be a novel promising vaccine adjuvant.
