CAJ | 학술논문

目的:探讨模拟失重环境下大鼠血浆蛋白质组变化特征.方法:健康成年雄性 Wistar 大鼠88只,按模拟失重时相随机分为11组,分别为6 h、12 h、1 d、2 d、3 d、5 d、1周、2周、3周、4周及0 h 组(对照组).采用尾悬吊法建立模拟失重动物模型,实验结束时取动物静脉血,利用表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术及 MB-WCX 磁珠检测大鼠静脉血浆蛋白质谱,应用 Ciphergen Protein Chip Software 3.2.0和 Biomarker Wizard 3.1.0软件分析数据.结果:发现18个重力敏感蛋白,其中在模拟失重早期,相对分子质量较小的6个蛋白的表达呈上调趋势,而相对分子质量较大的12个蛋白的表达则逐渐下调;在模拟失重后期(悬尾2~3周后),上述蛋白的表达均呈回归趋势.结论:模拟失重环境对大鼠静脉血浆蛋白质谱产生明显影响,研究重力敏感蛋白对进一步揭示失重对机体的影响及机制具有重要意义,并对医监医保可能有一定的价值.
목적:탐토모의실중배경하대서혈장단백질조변화특정.방법:건강성년웅성 Wistar 대서88지,안모의실중시상수궤분위11조,분별위6 h、12 h、1 d、2 d、3 d、5 d、1주、2주、3주、4주급0 h 조(대조조).채용미현조법건립모의실중동물모형,실험결속시취동물정맥혈,이용표면증강격광해흡전리비행시간질보(SELDI-TOF-MS)기술급 MB-WCX 자주검측대서정맥혈장단백질보,응용 Ciphergen Protein Chip Software 3.2.0화 Biomarker Wizard 3.1.0연건분석수거.결과:발현18개중력민감단백,기중재모의실중조기,상대분자질량교소적6개단백적표체정상조추세,이상대분자질량교대적12개단백적표체칙축점하조;재모의실중후기(현미2~3주후),상술단백적표체균정회귀추세.결론:모의실중배경대대서정맥혈장단백질보산생명현영향,연구중력민감단백대진일보게시실중대궤체적영향급궤제구유중요의의,병대의감의보가능유일정적개치.
Objective: To investigate the effects of the simulated weightlessness on the proteomic profiles of plas?ma in rats. Methods: Eighty-eight male adult Wistar rats were randomly divided into 11 groups according to the durations of simulated weightlessness, suspended for 6 h, 12 h, 1 d, 2 d, 3 d, 5 d, 1 week, 2 week, 3 week, 4 week and 0 h(control). Tail-suspension technique was used to simulate the weightlessness animal model. The rats were sacrificed and the venous blood samples were taken in each group at the end of the experiment. The pro?teomic profiles of the blood plasma were detected by the surface-enhanced laser desorption ionization time of flight mass spectrometry(SELDI-TOF-MS) and MB-WCX beads. All data were analyzed with the Ciphergen Pro?tein Chip Software 3.2.0 and Biomarker Wizard 3.1.0. Results: Eighteen peaks of gravity-sensitive proteins were discovered, and 6 proteins with low molecular weight were up-regulated in the early phase of the simulated weight?lessness, while 12 proteins with higher molecular weight showed downward trend on the contrary. Interestingly, a reversion to the standard levels was noticed in all the proteins of the rat blood plasma in the late phase(2 to 3 week after tail-suspension) of the simulated weightlessness. Conclusion: Simulated weightlessness could induce al?terations of proteomic profiles of rat plasma, and detection of the gravity-sensitive proteins could provide useful messages to disclose the mechanism of weightlessness impacts on the body and may also be meaningful in the as?tronaut medical surveillance.