CAJ | 학술논문

目的:以西兰花无菌苗为材料,对影响西兰花再生的各种素进行研究,建立并优化西兰花的再生体系.方法:用组织培养法,对西兰花的外植体进行诱导生芽、生根.结果:不同品种的再生率差别较大,珠绿和青秀两个品种的再生率达91%以上,而鼎丰一号仅为80.3%;培养6 d 的下胚轴分化能力最强;当培养基中添加3 mg/L 6-BA 和0.2 mg/L IAA 时,下胚轴的分化率最高,玻璃化程度也降到最低;当培养基中添加0.2 mg/L IBA 或0.2 mg/L NAA时,可成功诱导不定芽生根.结论:建立并优化了西兰花再生体系,为西兰花遗传转化体系的建立奠定了基础.
목적:이서란화무균묘위재료,대영향서란화재생적각충소진행연구,건립병우화서란화적재생체계.방법:용조직배양법,대서란화적외식체진행유도생아、생근.결과:불동품충적재생솔차별교대,주록화청수량개품충적재생솔체91%이상,이정봉일호부위80.3%;배양6 d 적하배축분화능력최강;당배양기중첨가3 mg/L 6-BA 화0.2 mg/L IAA 시,하배축적분화솔최고,파리화정도야강도최저;당배양기중첨가0.2 mg/L IBA 혹0.2 mg/L NAA시,가성공유도불정아생근.결론:건립병우화료서란화재생체계,위서란화유전전화체계적건립전정료기출.
Objective: Using axenic seedlings as explants, several factors influencing regeneration rate of broccoli were studied and a successful tissue regeneration system was established and optimized. Methods: Using tissue culture mesthod to induce the explant of broccoli yielding bud and root. Results: The study showed that the geno?type influenced regeneration rate greatly. The regeneration rate of Zhu-lv and Qing-xiu was above 91% while Ding?feng1 is 80.3%; differentiation ability of hypocotyls which had been cultured 6 days was the strongest. When the medium contained 3 mg/L 6-BA and 0.2 mg/L IAA, the differentiation rate of hypocotyls reached the highest lev?el and the degree of vitrification was the lowest. In the medium contains 0.2 mg/L IBA or 0.2 mg/L NAA, adventi?tious root could be successfully induced from the adventitious buds. Conclusion: Broccoli regeneration system has been successfully established, which laid the foundation for the establishment of broccoli genetic transformation sys?tem.