CAJ | 학술논문

瞬时表达技术是一种获得目的基短暂的高水平表达的技术.与稳定表达相比,瞬时表达所需时间短,但未将外基整合到宿主植物染色体中,不能稳定遗传给下一代.我们简要综述了植物瞬时表达技术的各种方法,介绍了各方法的理、技术流程、影响素及其应用.关其应用,主要分析了生物制剂的合成、转录元件和转录子的克隆与分析、基沉默、亚细胞定位、蛋白互作分析、抑制子功能鉴定、选择性剪接调控、植物品种的改良和选育等方面.
순시표체기술시일충획득목적기단잠적고수평표체적기술.여은정표체상비,순시표체소수시간단,단미장외기정합도숙주식물염색체중,불능은정유전급하일대.아문간요종술료식물순시표체기술적각충방법,개소료각방법적리、기술류정、영향소급기응용.관기응용,주요분석료생물제제적합성、전록원건화전록자적극륭여분석、기침묵、아세포정위、단백호작분석、억제자공능감정、선택성전접조공、식물품충적개량화선육등방면.
Transient expression is a technology to highly express the target gene in a short time. Compared with stable expression, transient expression is speedy. On the other hand, exogenous gene doesn't integrate into the host chromosome, so it won't be inherited. We briefly introduced the main methods of plant transient expression technology, including their principles, technique procedures, influencing factors, and applications. This technology could be widely employed in the synthesis of many plant derived biologicals, clone and analysis of the transcrip?tion components and transcription factors, gene silence, observation of protein subcellular localization and interac?tion, identification of the inhibitor's function, study of alternative splicing, as well as in the plant improvement and breeding, etc.