CAJ | 학술논문

目的研究程序性死亡因子-1(programmed death 1,PD-1)与慢性乙型病毒性肝炎病理的相关性,探讨PD-1在慢性HBV感染病情进展中的作用.方法对84例慢性乙型病毒性肝炎患者行肝组织活检,肝组织标本经10%福尔马林液固定、石蜡包埋切片后,以HE染色评判肝组织炎症活动度(G)和肝纤维化程度(S).采用免疫组织化学染色SP法检测肝组织中PD-1的表达,以红色为阳性表达区域,每张切片在高倍镜下(400×)选取5个不连续视野进行图像采集,采用Image-Pro Plus 6.0图像分析系统进行测量分析,以积分光密度(integral optical density,IOD)值表示PD-1在肝组织中的表达水平.结果 PD-1表达强度与肝组织炎症活动度及纤维化程度均呈正相关,相关系数分别为0.898(P<0.001)、0.700(P<0.001).肝组织炎症活动度G0~G4级组间,PD-1的表达差异均有统计学意义(P<0.05).肝组织纤维化S2期与S3期、S3期与S4期间PD-1的表达,差异无统计学意义(P >0.05),其余各纤维化分期之间PD-1的表达,差异均有统计学意义(P<0.05).结论肝组织内的PD-1表达水平与CHB患者肝脏炎症及纤维化发生、进展呈正相关.测定PD-1的IOD值有助于辅助评判肝组织炎症活动度及肝纤维化程度,可以作为评判肝炎病理情况的参考指标.
목적연구정서성사망인자-1(programmed death 1,PD-1)여만성을형병독성간염병리적상관성,탐토PD-1재만성HBV감염병정진전중적작용.방법대84례만성을형병독성간염환자행간조직활검,간조직표본경10%복이마림액고정、석사포매절편후,이HE염색평판간조직염증활동도(G)화간섬유화정도(S).채용면역조직화학염색SP법검측간조직중PD-1적표체,이홍색위양성표체구역,매장절편재고배경하(400×)선취5개불련속시야진행도상채집,채용Image-Pro Plus 6.0도상분석계통진행측량분석,이적분광밀도(integral optical density,IOD)치표시PD-1재간조직중적표체수평.결과 PD-1표체강도여간조직염증활동도급섬유화정도균정정상관,상관계수분별위0.898(P<0.001)、0.700(P<0.001).간조직염증활동도G0~G4급조간,PD-1적표체차이균유통계학의의(P<0.05).간조직섬유화S2기여S3기、S3기여S4기간PD-1적표체,차이무통계학의의(P >0.05),기여각섬유화분기지간PD-1적표체,차이균유통계학의의(P<0.05).결론간조직내적PD-1표체수평여CHB환자간장염증급섬유화발생、진전정정상관.측정PD-1적IOD치유조우보조평판간조직염증활동도급간섬유화정도,가이작위평판간염병리정황적삼고지표.
@@@@Objective To study the correlationship between the expression of programmed death 1 (PD-1) and the pathology of chronic hepatitis B, and explore the role of PD-1 in chronic HBV infection. Methods Liver biopsies were performed in all 84 patients with chronic hepatitis B, the liver tissues were fixed by 10% formalin and paraffin-embedded sections were stained by HE, and the hepatic inflammatory activity grade and fibrosis stages were evaluated. Immunohistochemical SP was applied to detect the expression of PD-1 in liver tissues. The positive expression means that red staining was found in five discontinuous visual fields under high magnification (400 ×). The results were analyzed by Image Pro Plus 6.0 software. The expression intensity of PD-1 in liver tissues were measured by integral optical density (IOD) titres. Results The expression of PD-1 was positively correlated with hepatic inflammatory activity grade and fibrosis stage, with correlation coefficient as 0.898 (P<0.001) and 0.700 (P<0.01) respectively. There were significant differences of PD-1 expression among the hepatic inflammatory activity grades of each groups (P<0.05). The expression of PD-1 were significantly different among the fibrosis stages of each group (P<0.05), except S2 and S3, S3 and S4 (P>0.05). Conclusions PD-1 expression in liver tissues are positively correlated with the hepatic inflammatory activity grade and fibrosis stage. Detection of PD-1 is worthy of evaluating hepatic inflammatory activity and fibrosis stages, meanwhile, it can provide valuable reference for assessment in hepatitic pathology.