CAJ | 학술논문

目的建立一种可供研究的兔脂肪肝及正常肝内肝炎性假瘤(IPL)模型,研究其超声表现,以便更好的服务临床.方法兔脂肪肝模型的建立:高脂饲料+5%的酒精喂养,4周;兔肝炎性假瘤的建立:用改良的注射方法将弗氏佐剂分别注入兔肝左右叶,分别于1周、2周、3周动态观察兔肝内病灶的超声表现,最后取标本做病理检查.结果⑴脂肪肝及正常肝内IPL的成功率均为100%.⑵常规超声及病理对照:①脂肪肝饮食4周后,肝实质回声细腻增强,病理表现为肝细胞不同程度的脂肪变性;②造模1周及2周的IPL病灶周围肝组织回声增强,增粗,病灶内部坏死区内残留不同程度未吸收的明胶海绵(造模时植入);③造模3周的IPL病灶周围肝实质回声均匀,病灶内部坏死区内不含有未吸收的明胶海绵,病理学上更接近临床IPL.结论通过观察兔脂肪肝及正常肝内IPL模型的动态形成过程,发现兔IPL建模3周为最佳,为进一步探讨IPL的形成机制及不同肝背景下IPL的影像学研究发挥了积极作用.
목적건립일충가공연구적토지방간급정상간내간염성가류(IPL)모형,연구기초성표현,이편경호적복무림상.방법토지방간모형적건립:고지사료+5%적주정위양,4주;토간염성가류적건립:용개량적주사방법장불씨좌제분별주입토간좌우협,분별우1주、2주、3주동태관찰토간내병조적초성표현,최후취표본주병리검사.결과⑴지방간급정상간내IPL적성공솔균위100%.⑵상규초성급병리대조:①지방간음식4주후,간실질회성세니증강,병리표현위간세포불동정도적지방변성;②조모1주급2주적IPL병조주위간조직회성증강,증조,병조내부배사구내잔류불동정도미흡수적명효해면(조모시식입);③조모3주적IPL병조주위간실질회성균균,병조내부배사구내불함유미흡수적명효해면,병이학상경접근림상IPL.결론통과관찰토지방간급정상간내IPL모형적동태형성과정,발현토IPL건모3주위최가,위진일보탐토IPL적형성궤제급불동간배경하IPL적영상학연구발휘료적겁작용.
Objective To establish a model carrying IPL in rabbits serving for clinical study and to analyse its ultrasound features. Methods Establishment of rabbit models of fatty liver: High fat diet+5% alcohol for 4 weeks;Establishment of rabbit models of IPL: Freund's complete adjuvant was injected with modified methods into the desirable portion of the liver in rabbits. In 1 week, 2 weeks, 3 weeks after the injection, all animals were examined on ultrasound and the images were analyzed. Then, the lesions were harvested for future pathological examination. Results ⑴The incidence rate of IPL was 100%.⑵Ultrasound and Pathology: ①Fatty liver diet for 4 weeks,liver echo is delicate enhancement, hepatic cells are fatty change in different degrees in pathology; ②Molding after 1and 2 weeks, liver echo is enhancement and thickening around the lesions. Necrotic area have gelatin sponge unabsorbed. ③Molding after 3weeks, liver echo is well-distributed around the lesions. Necrotic area don't have gelatin sponge, and pathology is close to clinical. Conclusions By dynamically observing the formation process of IPL rabbit model of fatty liver and normal liver. Fand the best time for IPLmodel is 3 weeks, It's better for inquire into formation mechanism of IPL. And imaging studies of the IPL in different hepatic background.