CAJ | 학술논문

目的研究植酸(IP6)对人胶质母细胞U118MG生长及增殖的影响并探讨其作用机制.方法体外培养U118MG细胞,使用MTT实验法检测植酸对U118MG细胞的增殖抑制影响;Western blot检测Bcl-2、Caspase-3、p53的蛋白水平.结果 MTT实验表明植酸具有抑制U118MG细胞生长的作用(P<0.05),并与剂量、时间呈正关系;植酸可抑制Bcl-2蛋白表达,且诱导Caspase-3、p53的活化,具有统计学差异(P<0.05).结论植酸具有抑制U118MG细胞增殖、诱导凋亡的作用,Bcl-2、Caspase-3、p53可能参与植酸对人胶质母细胞U118MG的诱导凋亡机制.
목적연구식산(IP6)대인효질모세포U118MG생장급증식적영향병탐토기작용궤제.방법체외배양U118MG세포,사용MTT실험법검측식산대U118MG세포적증식억제영향;Western blot검측Bcl-2、Caspase-3、p53적단백수평.결과 MTT실험표명식산구유억제U118MG세포생장적작용(P<0.05),병여제량、시간정정관계;식산가억제Bcl-2단백표체,차유도Caspase-3、p53적활화,구유통계학차이(P<0.05).결론식산구유억제U118MG세포증식、유도조망적작용,Bcl-2、Caspase-3、p53가능삼여식산대인효질모세포U118MG적유도조망궤제.
Objective In order to study the antiproliferative effects on Human glioma cells(U118MG) induced by phytic acid(IP6) and to shed new light on the mechanism of action. Methods We use the U118MG human glioma cell line as a model and examined the antiproliferation effects on U118MG cells treated with IP6 by MTT reduction assays. Western blot test Bcl-2, Caspase-3, p53 protein level. Results Our results indicated that IP6 induces antiproliferative effects in a concentration-dependent manner(P<0.05). Furthermore, IP6 inhibited the expression of Bcl-2 protein followed by activation of Caspase-3 and p53(P<0.05). Conclusions Accordingly, IP6 could inhibit U118MG cell proliferation and induce cell apoptosis. Bcl-2, Caspase-3, and p53 proteins may be involved in apoptosis mechanism on U118MG cells induced by IP6.