CAJ | 학술논문

目的观察大鼠二次脑损伤合并海水浸泡后脑组织病理改变.方法利用改良二次脑损伤动物模型,对创伤处进行持续海水浸泡,分别在1、3、6、12、48h对挫伤侧海马区取材,固定于10%福尔马林液中,作HE染色,光镜下观察脑组织病理改变.结果二次脑损伤合并海水浸泡组出现脑水肿早于单纯二次脑损伤组,HE染色病理学改变观察表明海水浸泡能在早期加重脑水肿,后期损伤更严重,呈网格状改变,单纯二次脑损伤组脑组织呈海绵状改变.结论对应时间窗下,SBI合并海水浸泡比单纯SBI严重,SBI合并海水浸泡伤程度发展更迅速.
목적관찰대서이차뇌손상합병해수침포후뇌조직병리개변.방법이용개량이차뇌손상동물모형,대창상처진행지속해수침포,분별재1、3、6、12、48h대좌상측해마구취재,고정우10%복이마림액중,작HE염색,광경하관찰뇌조직병리개변.결과이차뇌손상합병해수침포조출현뇌수종조우단순이차뇌손상조,HE염색병이학개변관찰표명해수침포능재조기가중뇌수종,후기손상경엄중,정망격상개변,단순이차뇌손상조뇌조직정해면상개변.결론대응시간창하,SBI합병해수침포비단순SBI엄중,SBI합병해수침포상정도발전경신속.
Objectives To observe the pathological impact of seawater on rats brain tissue after secondary brain insults. Methods Modified rats models of secondary brain insults, in which the damage brain tissues were subjected to immersion with fresh seawater continuously. Tissue sampling was performed at the brain injury sites of Hippocampus. Path-ological changes of traumatic brain edema was observed by hematoxylin and eosin method at designed time points(1h, 3h, 6h, 12h, 48h). Results The brain edema of SBI with seawater immersion is earlier and much serious than SBIgroups, Pathological change by HE method indicated that preinjury traumatic edema was aggravated as a result of seawater immersion and became grid changed not the same as Spongiform change of SBI group. Conclusion SBI with seawater immersion group is more serious than SBI group under the corresponding time window, the deteriorism of traumatic condition in SBI with seawater immersion became more serious quickly.