中国中西医结合外科杂志
中國中西醫結閤外科雜誌
중국중서의결합외과잡지
CHINESE JOURNAL OF SURGERY OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2013年
2期
149-152
,共4页
姜黄素%奥沙利铂%结肠癌%NF-κB%XIAP
薑黃素%奧沙利鉑%結腸癌%NF-κB%XIAP
강황소%오사리박%결장암%NF-κB%XIAP
Curcum%Oxaliplatin%colorectal cancer%XIAP%NF-κB
目的:探讨姜黄素增强奥沙利铂对肠癌HT-29细胞裸鼠移植瘤的诱导凋亡作用及其机制.方法:建立耐药肠癌HT-29细胞的裸鼠皮下移植瘤模型,将荷瘤鼠随机分为Con组(生理盐水)、Oxa组(奥沙利铂7.5 mg/kg)、Cur组(姜黄素50 mg/kg)和Oxa+Cur组(联合用药,奥沙利铂7.5 mg/kg和姜黄素50 mg/kg).腹腔注射给药,每3 d 1次,共8次.给药后测量肿瘤体积.免疫组织化学法检测肿瘤组织细胞增殖子(Ki-67)、凋亡抑制子(XIAP)和核转录子(NF-κ B)的蛋白表达.RT-PCR检测NF-κB和XIAP mRNA的表达.结果: Oxa+Cur组肿瘤体积和质量明显小其他各组.与Con组相比较,Oxa组、Cur组和Oxa+Cur组的Ki-67蛋白表达显著下降;与Oxa组和Cur组相比较,Oxa+Cur组的Ki-67蛋白表达显著下降.与Con组相比较, Oxa组、Cur组和Oxa+Cur组的NF-κB和XIAP蛋白和mRNA的表达显著下降;与Oxa组和Cur组相比较,Oxa+Cur组的NF-κB和XIAP蛋白和mRNA的表达显著下降.结论:姜黄素可以通过下调Ki-67和NF-κB 及XIAP基表达,增强奥沙利铂对肠癌HT-29细胞移植瘤的抑制细胞增殖和诱导凋亡作用.
目的:探討薑黃素增彊奧沙利鉑對腸癌HT-29細胞裸鼠移植瘤的誘導凋亡作用及其機製.方法:建立耐藥腸癌HT-29細胞的裸鼠皮下移植瘤模型,將荷瘤鼠隨機分為Con組(生理鹽水)、Oxa組(奧沙利鉑7.5 mg/kg)、Cur組(薑黃素50 mg/kg)和Oxa+Cur組(聯閤用藥,奧沙利鉑7.5 mg/kg和薑黃素50 mg/kg).腹腔註射給藥,每3 d 1次,共8次.給藥後測量腫瘤體積.免疫組織化學法檢測腫瘤組織細胞增殖子(Ki-67)、凋亡抑製子(XIAP)和覈轉錄子(NF-κ B)的蛋白錶達.RT-PCR檢測NF-κB和XIAP mRNA的錶達.結果: Oxa+Cur組腫瘤體積和質量明顯小其他各組.與Con組相比較,Oxa組、Cur組和Oxa+Cur組的Ki-67蛋白錶達顯著下降;與Oxa組和Cur組相比較,Oxa+Cur組的Ki-67蛋白錶達顯著下降.與Con組相比較, Oxa組、Cur組和Oxa+Cur組的NF-κB和XIAP蛋白和mRNA的錶達顯著下降;與Oxa組和Cur組相比較,Oxa+Cur組的NF-κB和XIAP蛋白和mRNA的錶達顯著下降.結論:薑黃素可以通過下調Ki-67和NF-κB 及XIAP基錶達,增彊奧沙利鉑對腸癌HT-29細胞移植瘤的抑製細胞增殖和誘導凋亡作用.
목적:탐토강황소증강오사리박대장암HT-29세포라서이식류적유도조망작용급기궤제.방법:건립내약장암HT-29세포적라서피하이식류모형,장하류서수궤분위Con조(생리염수)、Oxa조(오사리박7.5 mg/kg)、Cur조(강황소50 mg/kg)화Oxa+Cur조(연합용약,오사리박7.5 mg/kg화강황소50 mg/kg).복강주사급약,매3 d 1차,공8차.급약후측량종류체적.면역조직화학법검측종류조직세포증식자(Ki-67)、조망억제자(XIAP)화핵전록자(NF-κ B)적단백표체.RT-PCR검측NF-κB화XIAP mRNA적표체.결과: Oxa+Cur조종류체적화질량명현소기타각조.여Con조상비교,Oxa조、Cur조화Oxa+Cur조적Ki-67단백표체현저하강;여Oxa조화Cur조상비교,Oxa+Cur조적Ki-67단백표체현저하강.여Con조상비교, Oxa조、Cur조화Oxa+Cur조적NF-κB화XIAP단백화mRNA적표체현저하강;여Oxa조화Cur조상비교,Oxa+Cur조적NF-κB화XIAP단백화mRNA적표체현저하강.결론:강황소가이통과하조Ki-67화NF-κB 급XIAP기표체,증강오사리박대장암HT-29세포이식류적억제세포증식화유도조망작용.
Objective To investigate the enhanced effect of oxaliplatin by Curcum on HT-29 cell xenograft on athymic. Methods The models of HT-29 cell xenograft on athymic mouse were established and random?ized to four groups with intraperitoneal (IP) injection of different drugs (group Con 0.9% sodium chloride), group Oxa (oxaliplatin,7.5 mg/kg), group Cur ( Curcum 50 mg/kg)and group Oxa+Cur (oxaliplatin 7.5 mg/kg and Cur?cum 50 mg/kg in combination). The drugs were injected once every 3 days, 8 times in all. The mice were sacri?ficed 1 week after the last injection. The tumor volume and tumor weight were measured during the drug thera?py. Immunohistochemistry (IHC) was performed to detect the expression of NF-κB, XIAP and Ki-67, RT-PCR was performed to detect the expression of NF-κB and XIAPmRNA. Results One week after the last adminis?tration, the mean tumor volume and tumor weight in group Oxa+Cur were significantly decreased as compared to the other groups. IHC analysis showed the expression of NF-κ B and XIAP were down regulated in Cur and Oxa+Cur groups as compared to the other two groups. The expression of Ki-67 was also down regulated signifi?cantly in Oxa+Cur group as compared to the other groups. RT-PCR analysis showed the expression of NF-κB and XIAP mRNA in Con and Oxa+Cur groups were down regulated significantly as compared to the other groups. Conclusion Curcum can enhance the anti-tumor effect of oxaliplatin on colorectal cancer xenograft. Downregulation of NF-κB and XIAP is possibly one of the mechanisms.
