CAJ | 학술논문

从溶藻弧菌中提取肽聚糖,以0.05、0.1、0.15 mg/mL 3种浓度为注射凡纳滨对虾后,测定血淋巴中的血细胞数目、酚氧化酶(PO)、超氧化物歧化酶(SOD)、一氧化氮合成酶(NOS)的活性和虾青素的含量,探讨溶藻弧菌肽聚糖对凡纳滨对虾虾青素含量、免疫指标、免疫保护率的影响.结果表明:注射不同质量浓度的溶藻弧菌肽聚糖都对血清中的血细胞数目、PO、SOD、NOS活性影响显著(p值﹤0.05),对虾青素的含量影响极显著(p值﹤0.01);虾青素的含量与血细胞数目、PO、NOS活性变化趋势无明显的相似性,与SOD活性变化有相似的变化趋势;0.05 mg/mL组的免疫保护率为40%,0.1 mg/mL组为53.33%,0.15 mg/mL组为66.67%.溶藻弧菌肽聚糖可有效地增强凡纳滨对虾非特异免疫力,提高对溶藻弧菌的抗病力,虾青素含量与SOD活性的高低变化趋势相似.
종용조호균중제취태취당,이0.05、0.1、0.15 mg/mL 3충농도위주사범납빈대하후,측정혈림파중적혈세포수목、분양화매(PO)、초양화물기화매(SOD)、일양화담합성매(NOS)적활성화하청소적함량,탐토용조호균태취당대범납빈대하하청소함량、면역지표、면역보호솔적영향.결과표명:주사불동질량농도적용조호균태취당도대혈청중적혈세포수목、PO、SOD、NOS활성영향현저(p치﹤0.05),대하청소적함량영향겁현저(p치﹤0.01);하청소적함량여혈세포수목、PO、NOS활성변화추세무명현적상사성,여SOD활성변화유상사적변화추세;0.05 mg/mL조적면역보호솔위40%,0.1 mg/mL조위53.33%,0.15 mg/mL조위66.67%.용조호균태취당가유효지증강범납빈대하비특이면역력,제고대용조호균적항병력,하청소함량여SOD활성적고저변화추세상사.
Peptidoglycan extracted from Vibrio alginolyticus was injected in Litopenaeus vannamei with different concentrations (0.05,0.1,0.15 mg/mL) to investigate the relationship among the level of astaxanthin, immune indicators and immune protection. The number of blood cells, the activity of phenol oxidase (PO) , superoxide dismutase (SOD),nitric oxide synthase (NOS) in the hemolymph and the level of astaxanthin were measured. The results showed that the number of blood cells, the activity of PO, SOD, NOS were improved significantly (P<0.05) and the level of astaxanthin was significantly higher than that of the control group(P <0.01). The relationship between the astaxanthin and immune indicators had no correlation except for the SOD. Immune protection rate of different concentrations reached 40%, 53.33%, and 66.67%. These results suggested that the different concentrations of Vibrio alginolyticus peptidoglycan could effectively enhance non-specific immunity and disease resistance of the Vibrio alginolyticus. The level of astaxanthin had the similar trend to the SOD activity.