CAJ | 학술논문

为探究湛江近海域海洋沉积物中含氯多环芳烃的生物学毒性效应,选取湛江近海域2个地点(TS和JSW)采集样品,以含氯多环芳烃为目标,制备沉积物提取物,将斑马鱼胚胎胚胎暴露于不同浓度的沉积物提取物中,观测供试斑马鱼胚胎的死亡和畸形情况.结果表明:TS采样点沉积物提取物的LC50为15.98 mg/mL(48 h)和14.41mg/mL(96 h),JSW采样点沉积物提取物的LC50为19.89 mg/mL(48 h)和16.36 mg/mL(96 h),且致死效应呈现浓度依赖关系;TS和JSW 2个采样点沉积物中含氯多环芳烃能导致斑马鱼胚胎卵黄囊水肿、心包水肿、脊椎弯曲等致畸效应,其致畸效应与暴露浓度基本呈浓度依赖关系.湛江近海域沉积含氯多环芳烃物提取物对斑马鱼胚胎具有明显的致死作用和致畸毒性.
위탐구담강근해역해양침적물중함록다배방경적생물학독성효응,선취담강근해역2개지점(TS화JSW)채집양품,이함록다배방경위목표,제비침적물제취물,장반마어배태배태폭로우불동농도적침적물제취물중,관측공시반마어배태적사망화기형정황.결과표명:TS채양점침적물제취물적LC50위15.98 mg/mL(48 h)화14.41mg/mL(96 h),JSW채양점침적물제취물적LC50위19.89 mg/mL(48 h)화16.36 mg/mL(96 h),차치사효응정현농도의뢰관계;TS화JSW 2개채양점침적물중함록다배방경능도치반마어배태란황낭수종、심포수종、척추만곡등치기효응,기치기효응여폭로농도기본정농도의뢰관계.담강근해역침적함록다배방경물제취물대반마어배태구유명현적치사작용화치기독성.
To study the bio-toxicity of chlorinated polycyclic aromatic hydrocarbons in Zhanjiang coastal sediment, the sediments from Zhanjiang industrial area and living area (TS and JSW) were collected and extracted focusing upon chlorinated polycyclic aromatic hydrocarbons, respectively. Then a range of zebrafish embryos were exposed to different concentrations of the sediment extracts for 96 h to investigate the motality, teratogenicity. The results showed that the extracts from TS and JSW sediment caused marked motality and teratogenicity to zebrafish embryos. The LC50 of the extract from TS were 15.98 mg/mL for 48 h and 14.41 mg/mL for 96h, and the LC50 of the extract from JSW were 19.89 mg/mL for 48h and 16.36 mg/mL for 96h, with a concentration depended relationship. In addition, the zebrafish embryos suffered from yolk sac edema, pericardium edema and spinal curvature, with a concentration depended relationship caused by the sediment extracts. It was concluded that the sediments collected from Zhanjiang offshore exhibited relatively strong bio-toxicity of mortality and teratogenicity to zebrafish embryo.