CAJ | 학술논문

目的观察大鼠实验性蛛网膜下腔出血(subarachnoid hemorrhage ,SAH)后下丘脑组织匀浆5-羟色胺(5-hy-droxytryptamine,5-HT)、去甲肾上腺素(Noradrenaline,NA)水平随时间变化情况,以及地塞米松(dexamethasone,DXM)不同给药途径对两者水平的影响.方法实验大鼠随机分为空白对照组、SAH组、腹腔给药组和脑池内给药组,按时间分为3、7、14、21d 4个亚组,采用高效液相色谱法(HPLC)测定下丘脑组织中5-HT和NA的含量.结果5-HT水平在SAH后3d开始上升,7d达到高峰,14d仍维持在较高水平,21d下降到接近3d时的水平;NA的水平从第3天开始上升,第7、14、21天,水平呈逐渐升高的改变,与5-HT变化的时间模式不一致.DXM治疗组中5-HT水平均较SAH组明显降低(均P<0.05),且两种用药途径间无明显差异(P>0.05);DXM治疗对NA水平影响不大(P>0.05).结论 SAH发生后,可导致下丘脑组织中5-HT和NA水平升高,可能与SAH后睡眠/觉醒异常的发生相关,但两者变化的时间模式不同.DXM可降低SAH后下丘脑组织中5-HT水平,可能是其促进清醒的机制之一.DXM脑池内给药是一种可选择的用药途径.
목적관찰대서실험성주망막하강출혈(subarachnoid hemorrhage ,SAH)후하구뇌조직균장5-간색알(5-hy-droxytryptamine,5-HT)、거갑신상선소(Noradrenaline,NA)수평수시간변화정황,이급지새미송(dexamethasone,DXM)불동급약도경대량자수평적영향.방법실험대서수궤분위공백대조조、SAH조、복강급약조화뇌지내급약조,안시간분위3、7、14、21d 4개아조,채용고효액상색보법(HPLC)측정하구뇌조직중5-HT화NA적함량.결과5-HT수평재SAH후3d개시상승,7d체도고봉,14d잉유지재교고수평,21d하강도접근3d시적수평;NA적수평종제3천개시상승,제7、14、21천,수평정축점승고적개변,여5-HT변화적시간모식불일치.DXM치료조중5-HT수평균교SAH조명현강저(균P<0.05),차량충용약도경간무명현차이(P>0.05);DXM치료대NA수평영향불대(P>0.05).결론 SAH발생후,가도치하구뇌조직중5-HT화NA수평승고,가능여SAH후수면/각성이상적발생상관,단량자변화적시간모식불동.DXM가강저SAH후하구뇌조직중5-HT수평,가능시기촉진청성적궤제지일.DXM뇌지내급약시일충가선택적용약도경.
@@@@Objective To investigate the levels of 5-hydroxytryptamine (5-HT) and norepinephrine (NA) in rat hypothala-mus after subarachnoid hemorrhage (SAH), and the effect of dexamethasone (DXM) on 5-HT and NA. Methods The rats were randomly divided into 4 groups:the blank, SAH control, SAH treated by intracisternal DXM and SAH treated with intraperitoneal DXM. The levels of 5-HT and NA were measured by high performance liquid chromatograph. Results The 5-HT in hypothala-mus tissue began to rise in 3d, reached to peak in 7d, remained at high level in 14d and went down to d3 level in 21d, which was consistent with the timing of delayed cerebral ischemic. The NA in hypothalamus tissue began to rise in 3d and kept rising in 7, 14 and 21d, which was not consistent with the mode of 5-HT. The levels of 5-HT were decreased after DXM treatment and there was no significant difference between intracisternal and intraperitoneal administration. There were no changes of NA levels after DXM treatment. Conclusion 5-HT and NA levels in hypothalamus are increased after SAH, which may be involved in abnormal-ity of sleep/wakefulness, but the time modes of 5-HT and NA are different. DXM may promote wakefulness by lowering 5-HT level in hypothalamus after SAH, and DXM can be administrated intracisternal y as an alternative way.