上海计量测试
上海計量測試
상해계량측시
SHANGHAI MEASUREMENT AND TESTING
2013年
2期
22-24
,共3页
超高效液相色谱串联质谱%阿维菌素类药物%植物性食品%残留
超高效液相色譜串聯質譜%阿維菌素類藥物%植物性食品%殘留
초고효액상색보천련질보%아유균소류약물%식물성식품%잔류
UPLC-MS/MS%avermectins%plant derived food%residue
提出超高效液相色谱串联质谱法测定植物性食品中阿维菌素类药物(阿维菌素、伊维菌素、多拉菌素、甲氨基阿维菌素苯甲酸盐和乙酰胺基阿维菌素)残留量的方法.用乙腈提取溶剂样品中的目标物,经碱性氧化铝固相萃取小柱或炭黑氨基柱净化,超高效液相色谱分离后用串联质谱方法测定.以5类代表性植物性食品样品为基体,分别进行了4μg/kg、20μg/kg 和100μg/kg 3个添加水平的5种目标化合物的加标回收实验,回收率分别为80.6%~99.8%,RSD 分别为0.22%~6.4%.该方法简单快速,并且方法的灵敏度、准确度和重复性均满足农药多残留分析的要求.
提齣超高效液相色譜串聯質譜法測定植物性食品中阿維菌素類藥物(阿維菌素、伊維菌素、多拉菌素、甲氨基阿維菌素苯甲痠鹽和乙酰胺基阿維菌素)殘留量的方法.用乙腈提取溶劑樣品中的目標物,經堿性氧化鋁固相萃取小柱或炭黑氨基柱淨化,超高效液相色譜分離後用串聯質譜方法測定.以5類代錶性植物性食品樣品為基體,分彆進行瞭4μg/kg、20μg/kg 和100μg/kg 3箇添加水平的5種目標化閤物的加標迴收實驗,迴收率分彆為80.6%~99.8%,RSD 分彆為0.22%~6.4%.該方法簡單快速,併且方法的靈敏度、準確度和重複性均滿足農藥多殘留分析的要求.
제출초고효액상색보천련질보법측정식물성식품중아유균소류약물(아유균소、이유균소、다랍균소、갑안기아유균소분갑산염화을선알기아유균소)잔류량적방법.용을정제취용제양품중적목표물,경감성양화려고상췌취소주혹탄흑안기주정화,초고효액상색보분리후용천련질보방법측정.이5류대표성식물성식품양품위기체,분별진행료4μg/kg、20μg/kg 화100μg/kg 3개첨가수평적5충목표화합물적가표회수실험,회수솔분별위80.6%~99.8%,RSD 분별위0.22%~6.4%.해방법간단쾌속,병차방법적령민도、준학도화중복성균만족농약다잔류분석적요구.
It was reporeted that residual amount of avermectins(abamectin, emamectin benzoate, ivermectin and doramectin) in plant derived food was determined by UPLC-MS/MS. By extraction with acetonitrile, Futher cleanup was performed on a Alumina B or GCB/NH2 SPE cartridge.The connent of avermectins was determined by MS/MS. Tests for recovery were made by addition of standard avermectins at three diffrent(5, 10 and 25 μg/kg) concentration levels to five kinds of plant food as matrixs. values of recovery found were in the range of 80.6 % ~ 99.8%. With values of RSD's in the range of 0.22 % ~ 6.4 %.The method is fast, simple and convenient.
