CAJ | 학술논문

目的研究 F0F1-ATPase 纳米生物传感器在肠出血性大肠埃希菌 O157:H7检测中的应用,开发高灵敏、特异性强的快速检测技术.方法利用免疫技术构建信号识别元件,根据 F0F1-ATPase 酶学机制建信号转化系统,建立免疫生物传感器,最后通过荧光扫描仪进行信号检测.本研究以食品中常见的大肠埃希菌(Escherichia coli)和沙门菌(Salmonella typhi)为干扰菌株进行了该方法的特异性研究.结果检测时间为4.5 h,102~104 cfu 呈现良好的梯度性和线性, R2=0.9818.空白对照组和102 cfu/孔均与104 cfu/孔组存在极显著性差异, P 值分均为 P =0.00<0.01.结论 F0F1-ATPase 纳米生物传感器对 O157:H7检测快速、灵敏、特异性好,有良好的应用前景.
목적연구 F0F1-ATPase 납미생물전감기재장출혈성대장애희균 O157:H7검측중적응용,개발고령민、특이성강적쾌속검측기술.방법이용면역기술구건신호식별원건,근거 F0F1-ATPase 매학궤제건신호전화계통,건립면역생물전감기,최후통과형광소묘의진행신호검측.본연구이식품중상견적대장애희균(Escherichia coli)화사문균(Salmonella typhi)위간우균주진행료해방법적특이성연구.결과검측시간위4.5 h,102~104 cfu 정현량호적제도성화선성, R2=0.9818.공백대조조화102 cfu/공균여104 cfu/공조존재겁현저성차이, P 치분균위 P =0.00<0.01.결론 F0F1-ATPase 납미생물전감기대 O157:H7검측쾌속、령민、특이성호,유량호적응용전경.
@@@@Objective To develop a high sensitivity and specificity method for the detection of Es-cherichia coli O157:H7 by F0F1-ATPase immunobiosensors. Methods An immune biosensor based on the in-terference of load to the F0F1-ATPase rotation indicated by the fluorescence fluctuation was constructed to de-tect this pathogen. The concentration targets pathogen was measured according to the relative fluoresent value of F0F1-ATPase biosensor tagged by F1300 E.coli(ATCC14028) and Salmonella(CMCC44001) were tested as interferential bacteria to study the specificity of this method to O157: H7. Results The results demonstrated a good linearity (R2 = 0.9818) between antigen concentration (range from102 cfu to 104cfu) and the fluorescence intensity. The detection signals of the samples containing 102 cfu/well and 104 cfu/well O157: H7 were much stronger than the signal from control sample (P<0.01). Conclusion Owing to its much higher sensibility and simplicity than other currently applied methods, it provides a promising future for its application in food source pathogen detection.