CAJ | 학술논문

目的建立婴幼儿配方乳粉中双酚 A(BPA)、双酚 F(BPF)、己烷雌酚、己烯雌酚、双烯雌酚、辛基酚、壬基酚7种酚类环境激素的液相色谱-串联四极杆质谱检测方法.方法以1%三氯乙酸和乙腈提取, HLB 固相萃取净化,由 C18液相色谱柱分离,以电喷雾离子源负离子模式,多反应监测(MRM)条件下对7种物质进行检测,基质加标标准曲线法定量.结果7种化合物检出限在0.5~5μg/kg,定量限从1.5~15μg/kg.7种物质从定量限到50 ng/mL 范围内线性关系良好.在三水平加标回收试验中,7种化合物的回收率在73.5%~97.9%之间, RSD 为5.6%~13.6%.结论本文对7种环境激素同时检测的方法具有准确、高效、灵敏、特异性强等优点,为食品安全风险监控方法提供参考依据.
목적건립영유인배방유분중쌍분 A(BPA)、쌍분 F(BPF)、기완자분、기희자분、쌍희자분、신기분、임기분7충분류배경격소적액상색보-천련사겁간질보검측방법.방법이1%삼록을산화을정제취, HLB 고상췌취정화,유 C18액상색보주분리,이전분무리자원부리자모식,다반응감측(MRM)조건하대7충물질진행검측,기질가표표준곡선법정량.결과7충화합물검출한재0.5~5μg/kg,정량한종1.5~15μg/kg.7충물질종정량한도50 ng/mL 범위내선성관계량호.재삼수평가표회수시험중,7충화합물적회수솔재73.5%~97.9%지간, RSD 위5.6%~13.6%.결론본문대7충배경격소동시검측적방법구유준학、고효、령민、특이성강등우점,위식품안전풍험감공방법제공삼고의거.
@@@@Objective To establish an analytical method for determination of 7 phenolic environmental hormones-Bisphenol A, Bisphenol F, Hexestrol, Diethylstilbestrol, Dienestrol, Octylphenol, and Nonylphenol in infant formulas by liquid chromatography-triple quadrupole mass spectrometry. Methods Samples were extracted with 1% trichloroacetic acid and acetonitrile and purified by HLB solid phase extraction (SPE). The 7 compounds were separated by C18 column and analyzed by LC-MS/MS under multiple reaction monitoring (MRM) mode via negative electrospray ionization, and qualified by curvilinear equations of spiking standard in matrix. Results The limits of determination (LOD) of 7 compounds were 0.5~5 μg/kg, and the limits of quan-titation were 1.5~15 μg/kg. The results showed a good linearity over the range of LOQ to 50 ng/mL. The aver-age recoveries of 7 compounds were from 73.5% to 97.9% with the relative standard deviations (RSDs) of 5.6%~13.6% by three-level spiking tests. Conclusion The reported method of analyzing 7 environmental hormones simultaneously has the advantages of accuracy, efficiency, sensitivity and specificity, which will pro-vide references for the risk monitoring of food safety.