CAJ | 학술논문

目的应用胶体金免疫层析技术,研制一种玉米等谷物中玉米赤霉烯酮残留免疫胶体金快速检测试剂板.方法将制备的羊抗鼠 IgG、人工结合抗原和金标抗体三者经过反复调试优化,以适宜浓度和包被量分别包被到硝酸纤维素膜和胶体金结合垫上.包被后的硝酸纤维素膜和样品垫、胶体金结合垫、吸水垫及其他辅料组装成试纸条.结果该试剂板的最低检测限为100μg/kg,整个检测过程仅15 min,与其他霉菌毒素无交叉反应.结论该试剂板可用于玉米赤霉烯酮残留快速检测.
목적응용효체금면역층석기술,연제일충옥미등곡물중옥미적매희동잔류면역효체금쾌속검측시제판.방법장제비적양항서 IgG、인공결합항원화금표항체삼자경과반복조시우화,이괄의농도화포피량분별포피도초산섬유소막화효체금결합점상.포피후적초산섬유소막화양품점、효체금결합점、흡수점급기타보료조장성시지조.결과해시제판적최저검측한위100μg/kg,정개검측과정부15 min,여기타매균독소무교차반응.결론해시제판가용우옥미적매희동잔류쾌속검측.
@@@@Objective To develop a rapid test kit for zearalenone(ZEN) by colloidal gold immunochromatography technology. Methods Goat anti-mouse IgG, artificial conjugated-antigen and gold-labeled monoclonal antibody were optimized by repeated tests, and then coated to nitrocellulose (NC) membrane and conjugate pad with appropriate concentration and spray volume. After that, the coated NC membrane, the sample pad, the colloidal gold conjugate pad, absorbent pads and other accessories were assembled into a test strip. Results The lowest detection limit of ZEN in corn was 100 μg/kg and the whole test time was about 15 min. The cross-activity percentages to other mycotoxins were less than 1%. Conclusion This kit can be used for testing the zearalenone in corn rapidly.