CAJ | 학술논문

目的建立快速检测和鉴别单增李斯特菌、绵羊李斯特菌、英诺克李斯特菌、威尔李斯特菌和格氏李斯特菌等5种常见李斯特菌的方法.方法采用 PCR-RFLP 技术,首先通过引物“Lis1A-Lis1B”对李斯特菌属 iap 基因进行扩增,扩增产物大小约1.4 kb,然后用限制性内切酶 Dde Ⅰ对 PCR 产物进行酶切,电泳观察具有种间特异性的酶切谱带进行鉴别.结果上述5种李斯特菌的 PCR 扩增产物经内切酶 Dde Ⅰ消化后,得到片段大小不同、具有种间差异的特异性酶切图谱.结论本实验建立的 PCR-RFLP 方法可以用于上述5种常见李斯特菌的快速检测和鉴定.
목적건립쾌속검측화감별단증리사특균、면양리사특균、영낙극리사특균、위이리사특균화격씨리사특균등5충상견리사특균적방법.방법채용 PCR-RFLP 기술,수선통과인물“Lis1A-Lis1B”대리사특균속 iap 기인진행확증,확증산물대소약1.4 kb,연후용한제성내절매 Dde Ⅰ대 PCR 산물진행매절,전영관찰구유충간특이성적매절보대진행감별.결과상술5충리사특균적 PCR 확증산물경내절매 Dde Ⅰ소화후,득도편단대소불동、구유충간차이적특이성매절도보.결론본실험건립적 PCR-RFLP 방법가이용우상술5충상견리사특균적쾌속검측화감정.
@@@@Objective To establish a method to detect and identify five type strains of Listeria species such as Listeria monocytogenes, L. ivanuii, L. innocua, L. welshimeri and L. grayi. Methods The 1.4 kb gene iap from all members of the genus Listeria was amplified by using specific primers “Lis1A-Lis1B”, then cut by restriction endonuclease DdeⅠ. The different Listeria species could be identified through the specific enzyme spectrum of electrophoresis. Results The PCR amplification product of the five Listeria species were di-gested by DdeⅠ, and different sizes of fragments and species-specific endonuclease map were gotten. Conclu-sion This method with PCR-RFLP can be used to detect and identify the five common Listeria species rap-idly.