CAJ | 학술논문

目的建立一种简单、准确的卵磷脂测定方法.方法采用 KOH 溶液将磷脂酰胆碱中的胆碱游离,加雷氏盐反应后,在波长525 nm 处,通过分光光度法测定胆碱的量.结果该方法在0.075~0.225 mmol 范围内线性关系良好,线性方程为 Y =3.6457X+0.026, R2=0.9987,方法的回收率在95%~103%之间,相对标准偏差小于1.0%,待测液在2.5 h 之内稳定,能够满足检测要求.结论本方法具有操作简单、准确性高、稳定性好、抗干扰性强的优点,可以用于卵磷脂产品中磷脂酰胆碱含量的测定.
목적건립일충간단、준학적란린지측정방법.방법채용 KOH 용액장린지선담감중적담감유리,가뢰씨염반응후,재파장525 nm 처,통과분광광도법측정담감적량.결과해방법재0.075~0.225 mmol 범위내선성관계량호,선성방정위 Y =3.6457X+0.026, R2=0.9987,방법적회수솔재95%~103%지간,상대표준편차소우1.0%,대측액재2.5 h 지내은정,능구만족검측요구.결론본방법구유조작간단、준학성고、은정성호、항간우성강적우점,가이용우란린지산품중린지선담감함량적측정.
@@@@Objective To establish a simple and accurate method for determination of phosphatidylcho-line. Methods Free choline was obtained from phosphatidylcholine by alkaline hydrolysis and was deter-mined by spectrohpotometry under the wavelength of 525 nm after reaction with Reinecke’s salt. Results The method showed a good linearity over the range of 0.075m mol~0.225m mol,and the linear equation was Y =3.6457X+0.026 with R2=0.9987. The recoveries was in range of 95%~103%, and the relative standard devia-tions (RSDs) were lower than 1%. The liquid was stable in 2.5h, which can meet the testing requirements. Conclusion This method is simple, accurate, stable, and anti-interference, and it can be used for the determi-nation of phosphatidylcholine from lecithin products.