CAJ | 학술논문

芽胞杆菌作为益生菌的一种,在工业、农业、食品及医疗等领域都有着重要的应用价值.基因敲除技术是研究基因功能的重要手段,利用该技术进行分子生物学研究有利于加深对芽胞杆菌生物防治作用机理和调控机制的认识.本文综述了利用同源重组和随机插入突变两种基因敲除方式进行基因敲除的原理、特点及在芽胞杆菌中研究应用现状.最后探讨了该技术存在的局限性,并提出展望.
아포간균작위익생균적일충,재공업、농업、식품급의료등영역도유착중요적응용개치.기인고제기술시연구기인공능적중요수단,이용해기술진행분자생물학연구유리우가심대아포간균생물방치작용궤리화조공궤제적인식.본문종술료이용동원중조화수궤삽입돌변량충기인고제방식진행기인고제적원리、특점급재아포간균중연구응용현상.최후탐토료해기술존재적국한성,병제출전망.
As a kind of probiotics, Bacillus was widely used in industrial, agriculture , medical treatment and public health fields. Gene knockout technology is an important mean for gene function research which is conductive to understand the biological control and regulation mechanisms in Bacillus. This paper reviews the principles and characteristics of two kinds of gene knockout technology methods which are homologous recombination and insertion mutation, and their application advance in Bacillu, discusses the limitation of gene knockout technology, finally prospects the research and development in the future.