CAJ | 학술논문

以阴离子天然大分子透明质酸(HA)和阳离子单体甲基丙烯酸二甲氨基乙酯(DM)组成带相反电荷的聚合物/单体复合体系, DM通过水相原位聚合可制备荷正电的聚甲基丙烯酸二甲氨基乙酯(PDM), PDM与HA间的静电作用可诱导两者在水溶液中进行自组装,得到HA/PDM复合胶体粒子.用傅里叶变换红外(FTIR)光谱仪对HA/PDM复合物结构进行了表征.用动态激光光散射(DLS)研究了HA与PDM复合体系在水溶液中的自组装行为,并表征了反应时间对HA/PDM复合胶体粒子粒径的影响.利用透射电镜(TEM)表征了胶体粒子的形貌.考察了溶液pH对胶体粒子粒径及zeta电位的影响,并对胶体粒子的乳化性能进行初步探索.结果表明:DM单体聚合前,无HA/DM复合物聚集体形成;而随着DM的逐步聚合, HA与PDM可通过静电作用逐渐组装形成球状HA/PDM复合胶体粒子,其粒径随反应时间延长逐渐减小并趋于稳定.同时,该复合胶体粒子具有pH敏感性和乳化性,乳化性能较纯HA和PDM有较大提高.
이음리자천연대분자투명질산(HA)화양리자단체갑기병희산이갑안기을지(DM)조성대상반전하적취합물/단체복합체계, DM통과수상원위취합가제비하정전적취갑기병희산이갑안기을지(PDM), PDM여HA간적정전작용가유도량자재수용액중진행자조장,득도HA/PDM복합효체입자.용부리협변환홍외(FTIR)광보의대HA/PDM복합물결구진행료표정.용동태격광광산사(DLS)연구료HA여PDM복합체계재수용액중적자조장행위,병표정료반응시간대HA/PDM복합효체입자립경적영향.이용투사전경(TEM)표정료효체입자적형모.고찰료용액pH대효체입자립경급zeta전위적영향,병대효체입자적유화성능진행초보탐색.결과표명:DM단체취합전,무HA/DM복합물취집체형성;이수착DM적축보취합, HA여PDM가통과정전작용축점조장형성구상HA/PDM복합효체입자,기립경수반응시간연장축점감소병추우은정.동시,해복합효체입자구유pH민감성화유화성,유화성능교순HA화PDM유교대제고.
The anionic biomacromolecule, hyaluronic acid (HA), and cationic monomer, 2-(dimethylamino) ethyl methacrylate (DM), were used to construct an opposing charge polymer/monomer complex. Positively charged poly[2-(dimethylamino)ethyl methacrylate] (PDM) was prepared when DM monomers polymerized in aqueous solution. Self-assembly between PDM and HA was driven by electrostatic interaction, and HA/PDM complex col oid particles were formed in the aqueous medium. The HA/PDM complex structure was characterized by Fourier transform-infrared (FTIR) spectroscopy. The self-assembly behavior between HA and PDM, and the influence of polymerization time on HA/PDM complex col oid particle size, were investigated by dynamic laser scattering (DLS). Morphologies of the col oid particles were characterized by transmission electron microscopy (TEM). The influence of pH on the size and zeta potential of the col oid particles was investigated, and the emulsification of the col oid particles was preliminarily explored. The results showed that no HA/DM complex aggregates were formed before the polymerization of DM monomers. Spherical HA/PDM particles were spontaneously formed through electrostatic interaction between HA and PDM, coinciding with the gradual polymerization of DM. Particle sizes gradual y decreased and stabilized with increasing polymerization time. The HA/PDM particles were pH sensitive and possessed improved emulsification properties compared with uncomplexed HA and PDM.