西南军医
西南軍醫
서남군의
JOURNAL OF MILITARY SURGEON IN SOUTHWEST CHINA
2013年
3期
241-243
,共3页
郑晓博%陈光宇%黎鹏武%蒋志伟%邹洪%文艺%周超%汤礼军
鄭曉博%陳光宇%黎鵬武%蔣誌偉%鄒洪%文藝%週超%湯禮軍
정효박%진광우%려붕무%장지위%추홍%문예%주초%탕례군
微量输液泵%逆行胰胆管注射%牛黄胆酸钠%大鼠%重症急性胰腺炎%造模
微量輸液泵%逆行胰膽管註射%牛黃膽痠鈉%大鼠%重癥急性胰腺炎%造模
미량수액빙%역행이담관주사%우황담산납%대서%중증급성이선염%조모
microinfusion pump%retrograde biliopancreatic duct injection%sodium taurocholate%rats%severe acute pancreatitis(SAP)
目的探讨微量输液泵应用于逆行胰胆管注射牛黄胆酸钠大鼠重症急性胰腺炎造模的优势.方法108只健康雄性wistar大鼠分为手推法组(A组)、微量输液泵法组(B组)和假手术组(C组),每组36只,其中18只大鼠用于观察24h生存率,其余18只大鼠分3、6、12h三个时间点,每时间点分配6只.A组采用手推法经肠壁穿刺逆行胰胆管注射5%牛黄胆酸钠造成重症急性胰腺炎模型,B组采用微量输液泵法,C组仅行开腹关腹手术;于处理后3、6、12h取血、胰腺组织,检测血清淀粉酶水平、胰腺组织病理组织学评分,并观察24h生存率.结果 A组和B组各时间段血清淀粉酶和胰腺病理组织学评分均显著高于C组,B组各时间段血清淀粉酶和胰腺病理组织学评分均显著高于A组,B组24h生存率显著低于A组.结论微量输液泵应用于逆行胰胆管注射牛黄胆酸钠大鼠重症急性胰腺炎造模具有可靠、简便、成功率高的优点,且病变均一性较好.
目的探討微量輸液泵應用于逆行胰膽管註射牛黃膽痠鈉大鼠重癥急性胰腺炎造模的優勢.方法108隻健康雄性wistar大鼠分為手推法組(A組)、微量輸液泵法組(B組)和假手術組(C組),每組36隻,其中18隻大鼠用于觀察24h生存率,其餘18隻大鼠分3、6、12h三箇時間點,每時間點分配6隻.A組採用手推法經腸壁穿刺逆行胰膽管註射5%牛黃膽痠鈉造成重癥急性胰腺炎模型,B組採用微量輸液泵法,C組僅行開腹關腹手術;于處理後3、6、12h取血、胰腺組織,檢測血清澱粉酶水平、胰腺組織病理組織學評分,併觀察24h生存率.結果 A組和B組各時間段血清澱粉酶和胰腺病理組織學評分均顯著高于C組,B組各時間段血清澱粉酶和胰腺病理組織學評分均顯著高于A組,B組24h生存率顯著低于A組.結論微量輸液泵應用于逆行胰膽管註射牛黃膽痠鈉大鼠重癥急性胰腺炎造模具有可靠、簡便、成功率高的優點,且病變均一性較好.
목적탐토미량수액빙응용우역행이담관주사우황담산납대서중증급성이선염조모적우세.방법108지건강웅성wistar대서분위수추법조(A조)、미량수액빙법조(B조)화가수술조(C조),매조36지,기중18지대서용우관찰24h생존솔,기여18지대서분3、6、12h삼개시간점,매시간점분배6지.A조채용수추법경장벽천자역행이담관주사5%우황담산납조성중증급성이선염모형,B조채용미량수액빙법,C조부행개복관복수술;우처리후3、6、12h취혈、이선조직,검측혈청정분매수평、이선조직병리조직학평분,병관찰24h생존솔.결과 A조화B조각시간단혈청정분매화이선병리조직학평분균현저고우C조,B조각시간단혈청정분매화이선병리조직학평분균현저고우A조,B조24h생존솔현저저우A조.결론미량수액빙응용우역행이담관주사우황담산납대서중증급성이선염조모구유가고、간편、성공솔고적우점,차병변균일성교호.
Objective To study the advantages of mircroinfusion pump applied in retrograde biliopancreatic duct injection of sodium taurocholate in the induction of severe acute pancreatitis(SAP)model in rats. Methods 108 healthy male wistar rats were randomly di-vided into 3 groups:Group A, Group B and Group C, 36 rats in each, 18 out of 36 were taken for survival rate observation and the other 18 for other index observation at the time point of 3h, 6h and 12h;hand bolus retrograde injection of sodium taurocholate into biliopan-creatic duct through duodenal wall was made in Group A to induce SAP model while microinfusion pump was applied in Group B;rats in Group C underwent sham operation(open and shut)only;blood samples and prancreatic tissue were collected at the time points of 3h, 6h and 12h, and serum amylase level was measured and histopathologic grading of the pancreatic tissue was made;the survival rates in 24 hours were observed. Results The serum amylase levels and the histopathologic grading scores of Group A and of Group B were obviously higher than those of Group C;the serum amylase levels and the histopathologic grading scores of Group B at each time point were obviously higher than those of Group A while its survival rate in 24 hours were obviously lower than those of Group A. Conclu-sions The application of microinfusion pump in retrograde biliopancreatic duct injection of sodium taurocholate is reliable, simple and of high rate of success in the induction of SAP model in rats and can provide better uniformity of lesions.