CAJ | 학술논문

目的建立攀枝花地区1~8岁儿童血清补体C3、C4含量的参考范围.方法应用美国贝克曼库尔特公司IMMAGE 800特种蛋白质分析仪,采用免疫速率比浊法对912例1~8岁健康儿童血清补体C3、C4含量进行检测,分成7个年龄段,对结果进行统计分析.结果攀枝花地区1~8岁健康儿童血清补体C3、C4含量在性别和各年龄段之间差别均无统计学意义(P>0.05),总的参考范围为C3:0.60~1.82g/L,C4:0.18~0.22g/L.同时与全国临床检验操作规程及厂家提供的参考范围相比较,差异均有统计学意义(P<0.05).结论全国临床检验操作规程及厂家提供的参考范围已不适应本地区1~8岁儿童血清补体C3、C4的参考范围.各地各实验室应建立各自实验室的参考范围;若存在性别、年龄组间差异,应分别制定性别、年龄各段各自的参考范围.
목적건립반지화지구1~8세인동혈청보체C3、C4함량적삼고범위.방법응용미국패극만고이특공사IMMAGE 800특충단백질분석의,채용면역속솔비탁법대912례1~8세건강인동혈청보체C3、C4함량진행검측,분성7개년령단,대결과진행통계분석.결과반지화지구1~8세건강인동혈청보체C3、C4함량재성별화각년령단지간차별균무통계학의의(P>0.05),총적삼고범위위C3:0.60~1.82g/L,C4:0.18~0.22g/L.동시여전국림상검험조작규정급엄가제공적삼고범위상비교,차이균유통계학의의(P<0.05).결론전국림상검험조작규정급엄가제공적삼고범위이불괄응본지구1~8세인동혈청보체C3、C4적삼고범위.각지각실험실응건립각자실험실적삼고범위;약존재성별、년령조간차이,응분별제정성별、년령각단각자적삼고범위.
Objective To establish the reference intervals of serum complement C3 ad C4 for 1 to 8 years old healthy children in Pan-zhihua area. Methods Immunoturbidimetry was applied in detecting serum complement C3 and C4 of 912 children of 1 to 8 years old with IMMAGE 800(Beckman-coulter company, USA)special protein analyzer;the results of 7 age stages were statistical analyzed. Re-sults The difference of sex and age in serum complement C3 and C4 among 1 to 8 years old healthy children in Panzhihua area was of no statistical significance(P>0.05), the reference intervals were as follows:C3:0.60~1.82g/L, C4:0.18~0.22g/L, which was of statisti-cal difference from those in“The National Clinical Test Regualation of Operation”and the reference intervals provided by the manufac-turers(P<0.05). Conclusions“The National Clinical Test Regulation of Operation”and the reference intervals provided by the manu-facturers are no longer suitable for the detection of serum complement C3 and C4 in 1 to 8 years old healthy children in Panzhihua area;each lab should set up its own reference intervals and reference intervals of sex and each age stage if there exists difference in sex and age.