CAJ | 학술논문

目的探讨鲜壁虎活性组分对人肝癌 BEL-7404细胞增殖及凋亡的影响.方法将鲜壁虎活性组分冻干粉配制为不同浓度的溶液,并作用于肝癌 BEL-7404细胞.以 MTT 法检测不同浓度的鲜壁虎活性组分对肝癌 BEL-7404细胞增殖的抑制作用,流式细胞术分析各组细胞的凋亡情况.结果不同浓度的鲜壁虎活性组分均对肝癌 BEL-7404细胞的增殖有抑制作用,且呈剂量-时间依赖效应,实验组的抑制率均较对照组显著提高(P<0.05).不同浓度的鲜壁虎活性组分均能诱导肝癌 BEL-7404细胞凋亡,高浓度组(2560滋g/ml)的作用更为明显(P<0.05).结论鲜壁虎活性组分可显著抑制BEL-7404细胞的增殖,并诱导其凋亡.
목적탐토선벽호활성조분대인간암 BEL-7404세포증식급조망적영향.방법장선벽호활성조분동간분배제위불동농도적용액,병작용우간암 BEL-7404세포.이 MTT 법검측불동농도적선벽호활성조분대간암 BEL-7404세포증식적억제작용,류식세포술분석각조세포적조망정황.결과불동농도적선벽호활성조분균대간암 BEL-7404세포적증식유억제작용,차정제량-시간의뢰효응,실험조적억제솔균교대조조현저제고(P<0.05).불동농도적선벽호활성조분균능유도간암 BEL-7404세포조망,고농도조(2560자g/ml)적작용경위명현(P<0.05).결론선벽호활성조분가현저억제BEL-7404세포적증식,병유도기조망.
@@@@Objective To investigate the effects of fresh Gecko active component on proliferation and apoptosis in human hepatocel-lular carcinoma cell line BEL-7404. Methods Solutions were prepared containing different concentrations of freeze-dried fresh Gecko active component powder,and these were added to BEL-7404 cultures.MTT assay was used to measure the antiproliferative effects of the different concentrations,and flow cytometry was used to assess the extent of apoptosis at the different concentrations. Results Fresh Gecko active component inhibited BEL-7404 proliferation relative to the control group(P<0.05)in a time- and dose-dependent manner.The component also induced apoptosis in BEL-7404 cells,with the highest concentration(2 560 μg/ml)showing statistically significant effects(P<0.05). Conclusion Fresh Gecko active component can efficiently inhibit cell proliferation and induce apoptosis in the human hepatocellular carcinoma cell line BEL-7404.