CAJ | 학술논문

目的初步探讨金花茶花朵中的天然成分对人食管鳞癌细胞的生长抑制作用及其诱导细胞凋亡的情况.方法用不同浓度的金花茶花朵的水提取物作用于人食管鳞癌 Eca109细胞.采用台盼蓝染色法检测金花茶花朵的水提取物对 Eca109细胞活力的影响,应用透射电镜观察金花茶花朵的水提取物对癌细胞亚结构变化的作用.结果金花茶花朵的水提取物可抑制人食管鳞癌 Eca109细胞的生长,其作用呈浓度和时间依赖性关系,并且能诱导细胞凋亡.结论金花茶花朵的水提取物能抑制人食管鳞癌细胞的增殖,并可诱导其凋亡,从而为金花茶作为食管癌的化学预防制剂提供实验依据.
목적초보탐토금화다화타중적천연성분대인식관린암세포적생장억제작용급기유도세포조망적정황.방법용불동농도적금화다화타적수제취물작용우인식관린암 Eca109세포.채용태반람염색법검측금화다화타적수제취물대 Eca109세포활력적영향,응용투사전경관찰금화다화타적수제취물대암세포아결구변화적작용.결과금화다화타적수제취물가억제인식관린암 Eca109세포적생장,기작용정농도화시간의뢰성관계,병차능유도세포조망.결론금화다화타적수제취물능억제인식관린암세포적증식,병가유도기조망,종이위금화다작위식관암적화학예방제제제공실험의거.
@@@@Objective To explore the ability of an aqueous extract of Camellia chrysantha(Hu)Tuyama to inhibit proliferation and induce apoptosis in human esophageal squamous cell carcinoma cells. Methods Eca109 cells were treated with aqueous extract of Camellia chrysantha (Hu)Tuyama at various concentrations,and then cell growth was assessed by trypan blue exclusion and cell morphology by transmission electron microscopy(TEM). Results Aqueous extract of Camellia chrysantha(Hu)Tuyama inhibited Eca109 growth in a dose-and time-dependent manner.The extract also induced apoptosis-like morphological changes in the cells. Conclusion These results suggest that aqueous extract of Camellia chrysantha(Hu)Tuyama inhibits cell growth and induces apoptosis in human esophageal squamous carcinoma cells.