CAJ | 학술논문

目的研究儿童复发性呼吸道乳头状瘤(juvenile onset recurrent respiratory papillomatosis,JORRP)的患儿首次发病及完全治愈时肿瘤组织中 HPV6型和 HPV11型病毒含量的表达变化以及其病毒感染率.方法收集从首次发病到完全治愈的 JORRP 患儿29例,分别取每例患儿首次发病及治愈时的标本,采用 qRT-PCR 法分别测定其首次发病及完全治愈时乳头状瘤组织标本中 HPV 病毒的含量.结果①首次发病组与治愈组 HPV6型与 HPV11型病毒 qRT-PCR 相对定量结果的差异均无统计学意义(P跃0.05).②首次发病组与治愈组 HPV6型病毒感染的总体阳性率相同(P跃0.05);HPV11型病毒感染的总体阳性率不同(P约0.05).结论①完全治愈组与首次发病组的 JORRP 患儿相比,HPV6型与HPV11型病毒量的表达并无明显变化.②首次发病组的患儿均有 HPV11型病毒感染,治愈组的患儿 HPV11型病毒感染率有所下降.
목적연구인동복발성호흡도유두상류(juvenile onset recurrent respiratory papillomatosis,JORRP)적환인수차발병급완전치유시종류조직중 HPV6형화 HPV11형병독함량적표체변화이급기병독감염솔.방법수집종수차발병도완전치유적 JORRP 환인29례,분별취매례환인수차발병급치유시적표본,채용 qRT-PCR 법분별측정기수차발병급완전치유시유두상류조직표본중 HPV 병독적함량.결과①수차발병조여치유조 HPV6형여 HPV11형병독 qRT-PCR 상대정량결과적차이균무통계학의의(P약0.05).②수차발병조여치유조 HPV6형병독감염적총체양성솔상동(P약0.05);HPV11형병독감염적총체양성솔불동(P약0.05).결론①완전치유조여수차발병조적 JORRP 환인상비,HPV6형여HPV11형병독량적표체병무명현변화.②수차발병조적환인균유 HPV11형병독감염,치유조적환인 HPV11형병독감염솔유소하강.
@@@@Objective To investigate the expression of human papilloma virus 6(HPV6)and HPV11 in juvenile onset recurrent respira-tory papilloma(JORRP)and examine differences in HPV load and infection rate at initial infection and after complete cure. Methods A total of 29 children with JORRP were analyzed by real-time fluorescent quantitative PCR to determine HPV load at initial infection and after complete cure. Results ① HPV6 and HPV11 load did not differ significantly from each other at initial infection or after complete cure(P﹥0.05).② The rate of HPV6 infection was similar at initial infection and after complete cure(P﹥0.05),while the overall rate of HPV11 infection was different(P﹤0.05). Conclusion ① HPV6 and HPV11 load did not differ significantly from each other at initial infec-tion or after complete cure.② Children had HPV11 virus at initial infection,and the rate of HPV11 infection declined after complete cure.