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生物材料%纳米生物材料%纳米Ag-SiO2%聚氨酯%体外%L929 细胞%细胞相对增殖率%细胞毒性%抗菌性能%MTT比色法%省级基金%生物材料图片文章生物材料%納米生物材料%納米Ag-SiO2%聚氨酯%體外%L929 細胞%細胞相對增殖率%細胞毒性%抗菌性能%MTT比色法%省級基金%生物材料圖片文章
생물재료%납미생물재료%납미Ag-SiO2%취안지%체외%L929 세포%세포상대증식솔%세포독성%항균성능%MTT비색법%성급기금%생물재료도편문장
biomaterials%nano-biological materials%nanoAg-SiO 2%polyurethane%in vitro%L929 cells%relative growth rate%cytotoxicity%antibacterial properties%MTT colorimetric method%provincial grants-supported paper%biomaterial photographs-containing paper

背景:为改善聚氨酯材料的抗菌性能,前期研究中合成了纳米Ag-SiO2目的:比较7种含不同质量分数纳米Ag-SiO聚氨酯材料.2方法:将纳米Ag-SiO聚氨酯材料的体外细胞毒性.2与聚氨酯以熔融共混方式制备成含纳米Ag-SiO2结果与结论:7种含不同质量分数纳米Ag-SiO ,质量分数分别为0,0.5%,1.0%,1.5%,2.0%,2.5%,5.0%的聚氨酯材料.以上述7种聚氨酯材料浸提液、高密度聚乙烯浸提液(阴性对照)、0.1%苯酚液浸提液(阳性对照)分别培养L929细胞24,48,72 h后,并设置试剂对照(含体积分数10%胎牛血清的RPMI 1640液体培养基)和空白对照(不加细胞,只加含体积分数10%胎牛血清的RPMI 1640液体培养基).采用MTT比色法定量检测各组细胞相对增殖率,并进行毒性反应分级;同时在显微镜下观察细胞形态.2聚氨酯材料组、试剂对照组、阴性对照组细胞贴壁良好,形态正常,胞体丰满,胞质、核质分布均匀,细胞相对增殖率均大于80%,毒性反应分级均为1级,且随着纳米Ag-SiO2质量分数的降低,体外细胞毒性渐小、生物相容性更好.随培养时间延长,阳性对照组细胞相对增殖率逐渐降低,72 h后细胞相对增殖率降至8.7%,与其他组比较差异有显著性意义(P <0.05),细胞萎缩、变圆、漂浮、片状脱落.表明7种含不同质量分数纳米Ag-SiO2聚氨酯材料均具有良好的体外细胞相容性,毒性反应分级均为1级,符合医用材料体外实验要求.
배경:위개선취안지재료적항균성능,전기연구중합성료납미Ag-SiO2목적:비교7충함불동질량분수납미Ag-SiO취안지재료.2방법:장납미Ag-SiO취안지재료적체외세포독성.2여취안지이용융공혼방식제비성함납미Ag-SiO2결과여결론:7충함불동질량분수납미Ag-SiO ,질량분수분별위0,0.5%,1.0%,1.5%,2.0%,2.5%,5.0%적취안지재료.이상술7충취안지재료침제액、고밀도취을희침제액(음성대조)、0.1%분분액침제액(양성대조)분별배양L929세포24,48,72 h후,병설치시제대조(함체적분수10%태우혈청적RPMI 1640액체배양기)화공백대조(불가세포,지가함체적분수10%태우혈청적RPMI 1640액체배양기).채용MTT비색법정량검측각조세포상대증식솔,병진행독성반응분급;동시재현미경하관찰세포형태.2취안지재료조、시제대조조、음성대조조세포첩벽량호,형태정상,포체봉만,포질、핵질분포균균,세포상대증식솔균대우80%,독성반응분급균위1급,차수착납미Ag-SiO2질량분수적강저,체외세포독성점소、생물상용성경호.수배양시간연장,양성대조조세포상대증식솔축점강저,72 h후세포상대증식솔강지8.7%,여기타조비교차이유현저성의의(P <0.05),세포위축、변원、표부、편상탈락.표명7충함불동질량분수납미Ag-SiO2취안지재료균구유량호적체외세포상용성,독성반응분급균위1급,부합의용재료체외실험요구.
@@@@BACKGROUND: We synthesized nanoAg-SiO2 OBJECTIVE: To compare the in vitro cytotoxicity of seven kinds of polyurethane materials with different content of nanoAg-SiO polyurethane during preliminary research for improvement of antibacterial property of polyurethane materials. 2 METHODS: We melted nanoAg-SiO . 2 into polyurethane to prepare polyurethane materials containing 0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5% and 5.0% nanoAg-SiO2, respectively. Then we prepared the leaching liquor of high density polyethylene (negative control), 0.1% phenol fluid (positive control) and these nanoAg-SiO2 RESULTS AND CONCLUSION: Cel relative growth rate was greater than 80% and the toxicity reaction ranked level 1 in al polyurethane groups, reagent control group and negative control group. In the above-mentioned groups, cells adhered to the cavity wal with normal appearance, plump soma and cytoplasm under microscope;in vitro cytotoxicity of these polyurethane materials reduced and their biocompatibility improved when the nanoAg-SiO polyurethane materials to culture L929 cells for 24, 48 and 72 hours. cells cultured in RPMI 1640 medium containing 10% fetal bovine serum served as reagent control group, and blank control group was also set. Then MTT colorimetric method was used to quantitatively detect cel relative growth rate and conduct toxicity reaction grading. Cel morphology was observed under microscope. 2 content decreased. Cel relative growth rate became lower (P < 0.05) as culture-time prolonged and reduced to 8.7% after 72 hours in the positive control group. cells cultured in positive control group drifted in the leaching liquor with their trophy and round shape. Seven kinds of polyurethane materials with different contents of nanoAg-SiO2 have good in vitro cel ular compatibility and comply with the requirements of in vitro experiments for medical materials with their toxicity reaction ranking level 1.