CAJ | 학술논문

背景:胶原作为脊髓组织工程的良好支架有利于神经细胞及神经纤维的黏附和生长,但机械性能较差,需要在制备时提高其基本性能,还应对种子细胞的生物学行为产生积极影响.目的:分析胶原纳米组织工程支架的性能,检测其对神经干细胞凋亡行为及相关基因表达的影响.方法:采用电纺丝技术制备纤维定向排列及非定向排列的胶原纳米纤维膜,并对其进行表征.将新生SD 大鼠脊髓源性神经干细胞分别与纤维定向排列及非定向排列的胶原纳米纤维膜共培养,并设置单独神经干细胞培养为对照,检测细胞凋亡情况及相关基因表达变化.结果与结论:定向及非定向胶原纳米纤维膜的直径及形貌均达到纳米组织工程支架标准,溶胀系数较高,孔隙率较高,力学性能佳.与对照组比较,两胶原纳米纤维膜组神经干细胞凋亡率明显降低(P <0.05),细胞凋亡相关基因中 bcl-2基因表达量明显增加,bax 及 Caspase-3基因表达明显下降;两胶原纳米纤维膜组神经干细胞凋亡率差异无显著性意义.表明新型胶原纳米组织工程支架性能良好,能够抑制细胞凋亡,从基因表达水平调节神经干细胞的凋亡行为.
배경:효원작위척수조직공정적량호지가유리우신경세포급신경섬유적점부화생장,단궤계성능교차,수요재제비시제고기기본성능,환응대충자세포적생물학행위산생적겁영향.목적:분석효원납미조직공정지가적성능,검측기대신경간세포조망행위급상관기인표체적영향.방법:채용전방사기술제비섬유정향배렬급비정향배렬적효원납미섬유막,병대기진행표정.장신생SD 대서척수원성신경간세포분별여섬유정향배렬급비정향배렬적효원납미섬유막공배양,병설치단독신경간세포배양위대조,검측세포조망정황급상관기인표체변화.결과여결론:정향급비정향효원납미섬유막적직경급형모균체도납미조직공정지가표준,용창계수교고,공극솔교고,역학성능가.여대조조비교,량효원납미섬유막조신경간세포조망솔명현강저(P <0.05),세포조망상관기인중 bcl-2기인표체량명현증가,bax 급 Caspase-3기인표체명현하강;량효원납미섬유막조신경간세포조망솔차이무현저성의의.표명신형효원납미조직공정지가성능량호,능구억제세포조망,종기인표체수평조절신경간세포적조망행위.
BACKGROUND: As a good material for spinal cord tissue engineering scaffold, col agen is conducive to adhesion and growth of nerve cells and nerve fibers. But it needs to be improved in the preparation process because of its poor mechanical properties, not only to improve its basic performance, but also to have a positive impact on the biological behavior of the seed cells. OBJECTIVE: To observe the properties of new nano-scaffolds for tissue engineering and to detect the changes of neural stem cel apoptosis and related gene expression affected by the nano-scaffold. METHODS: Aligned and randomly oriented nanofibrous scaffolds were made of col agen by using electrospinning technology. Superficial morphous, porosity, mechanical property, swel ing coefficient, degradation disposition were tested. Spinal cord derived neural progenitor cells were cultured and identified, and then the cells were cultured on aligned and randomly oriented col agen nanofibrous scaffolds. cells cultured under normal conditions served as control group. The changes of neural stem cel apoptosis and related gene expression were tested. RESULTS AND CONCLUSION: Superficial morphous of electrospun aligned and randomly oriented col agen nanofibrous scaffolds were in accordance with contrivable requisition, their porosities were supernal, mechanical properties were fine, swel ing coefficients were satisfactory. Compared with the control group, apoptosis rate of cells cultured on aligned and randomly oriented col agen nano fibrous scaffolds decreased significantly (P < 0.05), expression level of apoptosis-related gene bcl-2 significantly increased, bax and Caspase-3 significantly decreased. There was no significant difference between the cells on aligned and randomly oriented col agen nano These findings indicate that properties and histocompatibility of new nano-scaffolds for tissue engineering are satisfactory, which can inhibit and regulate cel apoptosis from the level of gene expression.