CAJ | 학술논문

生物材料%材料生物相容性%β-磷酸三钙%聚乙烯醇%水凝胶%人工角膜%体外细胞毒性%急性全身毒性%皮内反应%迟发型超敏反应%其他基金生物材料%材料生物相容性%β-燐痠三鈣%聚乙烯醇%水凝膠%人工角膜%體外細胞毒性%急性全身毒性%皮內反應%遲髮型超敏反應%其他基金
생물재료%재료생물상용성%β-린산삼개%취을희순%수응효%인공각막%체외세포독성%급성전신독성%피내반응%지발형초민반응%기타기금
biomaterials%material biocompatibility%beta-tricalcium phosphate%poly (vinyl alcohol)%hydrogel%artificial cornea%in vitro cytotoxicity%acute systemic toxicity%intradermal reaction%delayed-type hypersensitivity%other grants-supported paper

背景:β-磷酸三钙/聚乙烯醇复合水凝胶具有高含水量、良好柔软性优点,有利于成纤细胞生长及胶原沉积,适用于做人工角膜裙边支架材料.目的:评价β-磷酸三钙/聚乙烯醇复合水凝胶人工角膜裙边支架材料的生物相容性.方法:①迟发型超敏反应:在豚鼠脊柱两侧皮内由头到尾分别注射 A 液(完全弗氏佐剂与生理盐水等体积混合液)、B 液(β-磷酸三钙/聚乙烯醇复合水凝胶浸提液或生理盐水或2-巯基苯并噻唑)、C 液(A 液与B 液等体积混合稳定性乳化剂),并进行局部诱导及激发.②急性全身性毒性实验:由昆明小鼠尾静脉分别注射生理盐水与β-磷酸三钙/聚乙烯醇复合水凝胶浸提液.③体外细胞毒性实验:分别以β-磷酸三钙/聚乙烯醇复合水凝胶浸提液、细胞培养液及含苯酚的细胞培养液培养 MRC-5细胞.④皮内反应:在兔脊柱皮内分别注射β-磷酸三钙/聚乙烯醇复合水凝胶生理盐水(或芝麻油)浸提液、生理盐水及芝麻油.结果与结论:β-磷酸三钙/聚乙烯醇复合水凝胶人工角膜裙边支架材料的迟发型超敏反应分级为0-1级,急性全身毒性实验结果为正常无症状,体外细胞毒性均为0级或1级,皮内反应为极轻微.表明β-磷酸三钙/聚乙烯醇复合水凝胶人工角膜裙边支架材料生物相容性指标达到生物植入医用材料的要求.
배경:β-린산삼개/취을희순복합수응효구유고함수량、량호유연성우점,유리우성섬세포생장급효원침적,괄용우주인공각막군변지가재료.목적:평개β-린산삼개/취을희순복합수응효인공각막군변지가재료적생물상용성.방법:①지발형초민반응:재돈서척주량측피내유두도미분별주사 A 액(완전불씨좌제여생리염수등체적혼합액)、B 액(β-린산삼개/취을희순복합수응효침제액혹생리염수혹2-구기분병새서)、C 액(A 액여B 액등체적혼합은정성유화제),병진행국부유도급격발.②급성전신성독성실험:유곤명소서미정맥분별주사생리염수여β-린산삼개/취을희순복합수응효침제액.③체외세포독성실험:분별이β-린산삼개/취을희순복합수응효침제액、세포배양액급함분분적세포배양액배양 MRC-5세포.④피내반응:재토척주피내분별주사β-린산삼개/취을희순복합수응효생리염수(혹지마유)침제액、생리염수급지마유.결과여결론:β-린산삼개/취을희순복합수응효인공각막군변지가재료적지발형초민반응분급위0-1급,급성전신독성실험결과위정상무증상,체외세포독성균위0급혹1급,피내반응위겁경미.표명β-린산삼개/취을희순복합수응효인공각막군변지가재료생물상용성지표체도생물식입의용재료적요구.
BACKGROUND: Beta-tricalcium phosphate/poly (vinyl alcohol) hydrogel (β-TCP/PVA-H) composite is a material with high water content, good softness, which is beneficial to the fibroblasts growth and col agen deposition. OBJECTIVE: To study the biocompatibility of β-TCP/PVA-H composite as an artificial cornea skirt material. METHODS: Delayed-type hypersensitivity: Both sides of the spinal skin of the guinea pig from beginning to end were injected with A solution (the mixture of complete Freund’s adjuvant and normal saline), B solution (extraction ofβ-TCP/PVA-H composite or normal saline or 2-Mercaptobenzothiazole), C solution (mixture of A and B solutions at equal volume), fol owed by local induction and inspire. The acute systemic toxicity test: Kunming mouse tail vein was injected with normal saline and β-TCP/PVA-H composite extraction. In vitro cytotoxicity assays: MRC-5 cells were cultured by β-TCP/PVA-H composite extraction, cel culture medium and phenol-containing cel culture medium. Intradermal reaction: The extracts of β-TCP/PVA-H composite, normal saline was injected intradermal y into the rabbit spine by normal saline (or sesame oil), normal saline and sesame oil. RESULTS AND CONCLUSION: The biocompatibility experiments of β-TCP/PVA-H composite showed that delayed-type hypersensitivity was graded 0-1, acute systemic toxicity test showed no symptoms, in vitro cytotoxicity was graded 0 or 1, and skin irritations were very slight. The experiments are agreed with the relative national standards, and β-TCP/PVA-H composite skirt as artificial cornea scaffold is a qualified medical material.