CAJ | 학술논문

生物材料%材料生物相容性%新鲜鹿骨%鹿脱蛋白松质骨%深低温冻干鹿脱蛋白松质骨%生物相容性%溶血实验%凝血实验%急性毒性实验%省级基金生物材料%材料生物相容性%新鮮鹿骨%鹿脫蛋白鬆質骨%深低溫凍榦鹿脫蛋白鬆質骨%生物相容性%溶血實驗%凝血實驗%急性毒性實驗%省級基金
생물재료%재료생물상용성%신선록골%록탈단백송질골%심저온동간록탈단백송질골%생물상용성%용혈실험%응혈실험%급성독성실험%성급기금
biomaterials%material biocompatibility%fresh deer bone%deer deproteinized cancel ous bone%deer free-dried deproteinized cancel ous bone%biocompatibility%hemolysis tests%coagulation tests%acute toxicity tests%provincial grants-supported paper

背景:经过脱蛋白去抗原处理的异种松质骨作为骨移植材料,具有天然的多孔结构、可塑性及一定的机械强度.目的:观察鹿脱蛋白松质骨的生物相容性.方法:①热源实验与急性毒性实验:将鹿新鲜骨、脱蛋白松质骨及深低温冻干脱蛋白松质骨浸提液分别注入家兔耳缘静脉与小鼠腹腔.②溶血实验:将兔血混悬液分别加入鹿新鲜骨、脱蛋白松质骨、深低温冻干脱蛋白松质骨、碳酸钠(阳性对照)、生理盐水(阴性对照)中.③凝血实验:将鹿新鲜骨、脱蛋白松质骨及深低温冻干脱蛋白松质骨分别加入兔正常混合血浆中.④肌袋实验:在小鼠大腿肌袋处分别植入新鲜鹿骨、鹿脱蛋白松质骨及深低温冻干脱蛋白松质骨.结果与结论:鹿脱蛋白松质骨及深低温冻干鹿脱蛋白松质骨无热源反应,未引起毒性、溶血及凝血反应,植入小鼠肌肉内后未发生排斥反应.新鲜鹿骨6种实验有轻度异常,但无动物死亡.表明鹿脱蛋白松质骨及深低温冻干鹿脱蛋白松质骨具有良好的生物相容性.
배경:경과탈단백거항원처리적이충송질골작위골이식재료,구유천연적다공결구、가소성급일정적궤계강도.목적:관찰록탈단백송질골적생물상용성.방법:①열원실험여급성독성실험:장록신선골、탈단백송질골급심저온동간탈단백송질골침제액분별주입가토이연정맥여소서복강.②용혈실험:장토혈혼현액분별가입록신선골、탈단백송질골、심저온동간탈단백송질골、탄산납(양성대조)、생리염수(음성대조)중.③응혈실험:장록신선골、탈단백송질골급심저온동간탈단백송질골분별가입토정상혼합혈장중.④기대실험:재소서대퇴기대처분별식입신선록골、록탈단백송질골급심저온동간탈단백송질골.결과여결론:록탈단백송질골급심저온동간록탈단백송질골무열원반응,미인기독성、용혈급응혈반응,식입소서기육내후미발생배척반응.신선록골6충실험유경도이상,단무동물사망.표명록탈단백송질골급심저온동간록탈단백송질골구유량호적생물상용성.
@@@@BACKGROUND: Antigen-extracted xenogeneic cancel ous bone as a kind of bone implant material has a natural porous structure, plasticity, and certain mechanical strength. OBJECTIVE: To study the biocompatibility of the deer deproteinized cancel ous bone. METHODS: Pyrogen test and acute toxicity test: Fresh deer bone, deproteinized cancel ous bone, and free-dried deproteinized cancel ous bone extracts were injected into the ear vein of rabbits and the abdominal cavity of mice. Hemolysis test: Rabbit mixed blood was added into the fresh deer bone, deproteinized cancel ous bone, and free-dried deproteinized cancel ous bone extracts, sodium carbonate (positive control), and normal saline (negative control). Coagulation test: Fresh deer bone, deproteinized cancel ous bone, and free-dried deproteinized cancel ous bone were placed into normal rabbit mixed blood. Long-term muscle test: Fresh deer bone, deproteinized cancel ous bone, and free-dried deproteinized cancel ous bone were implanted into the thigh muscle pouches of mice. RESULTS AND CONCLUSION: Deer deproteinized cancel ous bone and free-dried deproteinized cancel ous bone had no pyrogen reaction, toxicity, hemolysis and hemopexis reactions, and did not result in rejection reaction after implantation to the thigh muscle pouches of mice. There was a mild abnormality for the fresh deer bone in pyrogen, coagulation, hemolysis and long-term muscle tests without death. These findings indicate that deer deproteinized cancel ous bone and free-dried deproteinized cancel ous bone have good biocompatibility.