CAJ | 학술논문

背景:前期实验已初步证实许旺细胞复合小肠黏膜下层及碱性成纤维细胞生长因子构建的人工神经具有体外神经活性、趋化性.目的:观察许旺细胞及小肠黏膜下层复合碱性成纤维细胞生长因子缓释微球修复周围神经缺损后神经传导的再通情况.方法:制作 SD 大鼠坐骨神经缺损模型,随机分组:实验组以许旺细胞及小肠黏膜下层复合碱性成纤维细胞生长因子缓释微球修复,阳性对照组以许旺细胞及小肠黏膜下层复合游离碱性成纤维细胞生长因子修复,阴性对照组以许旺细胞及小肠黏膜下层修复,空白对照组以自体神经修复.结果与结论:术后16周实验组再生神经纤维数目,DiI 示踪标记的阳性神经元数量、S-100及神经细丝蛋白的阳性表达率、髓鞘及再生轴突的超微结构恢复、神经传导速度及复合动作电位的改善均优于阳性对照组与阴性对照组(P <0.05).表明许旺细胞复合小肠黏膜下层及碱性成纤维细胞生长因子缓释微球构建的人工神经可重建坐骨神经缺损后的神经传导通路.
배경:전기실험이초보증실허왕세포복합소장점막하층급감성성섬유세포생장인자구건적인공신경구유체외신경활성、추화성.목적:관찰허왕세포급소장점막하층복합감성성섬유세포생장인자완석미구수복주위신경결손후신경전도적재통정황.방법:제작 SD 대서좌골신경결손모형,수궤분조:실험조이허왕세포급소장점막하층복합감성성섬유세포생장인자완석미구수복,양성대조조이허왕세포급소장점막하층복합유리감성성섬유세포생장인자수복,음성대조조이허왕세포급소장점막하층수복,공백대조조이자체신경수복.결과여결론:술후16주실험조재생신경섬유수목,DiI 시종표기적양성신경원수량、S-100급신경세사단백적양성표체솔、수초급재생축돌적초미결구회복、신경전도속도급복합동작전위적개선균우우양성대조조여음성대조조(P <0.05).표명허왕세포복합소장점막하층급감성성섬유세포생장인자완석미구구건적인공신경가중건좌골신경결손후적신경전도통로.
BACKGROUND: To combine Schwann cells (SCs) and smal intestinal submucosa (SIS) with basic fibroblast growth factor (bFGF) is a feasible way to construct artificial nerve that possesses nerve activity and chemotaxis in vitro. OBJECTIVE: To observe the recanalization of neural pathways after repairing peripheral defects with bFGF control ed release microspheres combined with SCs and SIS. METHODS: Models of sciatic nerve defects were established in Sprague-Dawley rats, and then randomized into:experimental group repaired with bFGF control ed release microspheres combined with SCs and SIS, positive control group with SCs and SIS combined with free bFGF, negative control group with SCs and SIS, and blank control group with autologous nerve. RESULTS AND CONCLUSION: The number of regenerated nerve fibers, number of DiI tracer-positive neurons, positive expression rate of S-100 and neurofilament protein, ultrastructure of regenerated myelin and axons, nerve conduction velocity and composite action potential in the experimental group were superior to those in the positive and negative control groups at 16 weeks after operation (P < 0.05). These findings indicate that bFGF control ed release microspheres combined with SCs and SIS can reconstruct the neural pathways fol owing sciatic nerve injury.