CAJ | 학술논문

背景:Cylex ImmuKnow 检测方法是惟一得到 FDA 认可的检测移植受者细胞免疫功能的方法,它直接反映细胞免疫的功能,具有灵敏度高、特异性强,结果量化等优点.目的:通过对肾移植后患者监测细胞免疫功能 iATP 值(intracel ular adenosine triphosphatei),分析细胞免疫功能与肾移植术后排斥或感染的关系.方法:140例肾移植受者采用 ImmuKnowTM-Cylex?方法检测细胞免疫功能,收集数据结合患者临床状态分为排斥组18例,感染组35例与稳定组87例,同时收集61份健康人群血样检测细胞免疫功能数据作对照.分析细胞免疫功能与肾移植患者移植后发生排斥与感染的关系.结果与结论:细胞免疫功能 iATP 值检测结果显示,感染组患者有71.4%(n=25)分布在低免疫区,同比对照组、稳定组与排斥组比例明显增高(P <0.05).感染组 iATP 值显著低于其他3组.说明感染与细胞免疫功能低下两者具有明显的相关性,细胞免疫功能测定对监测肾移植后感染具有显著意义.提示ImmuKnowTM-Cylex?方法测定细胞免疫功能为肾移植后并发感染的诊断与治疗提供可靠客观依据,但与肾移植后排斥反应的发生未见明显的相关性,有待于大样本的实验证实.
배경:Cylex ImmuKnow 검측방법시유일득도 FDA 인가적검측이식수자세포면역공능적방법,타직접반영세포면역적공능,구유령민도고、특이성강,결과양화등우점.목적:통과대신이식후환자감측세포면역공능 iATP 치(intracel ular adenosine triphosphatei),분석세포면역공능여신이식술후배척혹감염적관계.방법:140례신이식수자채용 ImmuKnowTM-Cylex?방법검측세포면역공능,수집수거결합환자림상상태분위배척조18례,감염조35례여은정조87례,동시수집61빈건강인군혈양검측세포면역공능수거작대조.분석세포면역공능여신이식환자이식후발생배척여감염적관계.결과여결론:세포면역공능 iATP 치검측결과현시,감염조환자유71.4%(n=25)분포재저면역구,동비대조조、은정조여배척조비례명현증고(P <0.05).감염조 iATP 치현저저우기타3조.설명감염여세포면역공능저하량자구유명현적상관성,세포면역공능측정대감측신이식후감염구유현저의의.제시ImmuKnowTM-Cylex?방법측정세포면역공능위신이식후병발감염적진단여치료제공가고객관의거,단여신이식후배척반응적발생미견명현적상관성,유대우대양본적실험증실.
BACKGROUND: Cylex ImmuKnow is the first and only U S Food and Drug Administration-cleared blood test to measure immune cel function, it can reflect the immune function of the cells directly, which is a valuable tool with good qualities of high sensitivity and specificity. OBJECTIVE: To investigate the correlation between immune cell function and the rejection and infection after renal transplantation through monitoring of immune function intracellular adenosine triphosphatei value. METHODS: We col ected 140 renal transplant patients. The immune cel function levels were measured by ImmuKnowTM-Cylex?. The patients were divided into three groups according to clinical status of patients, namely, rejection group (n=18), infection group (n=35) and stability group (n=87). In addition, the cel ular immune function data in the blood of 61 samples from healthy people were col ected as control group. Then the correlation of the immune cel function and infection to rejection after renal transplantation was analyzed. RESULTS AND CONCLUSION: The measurement of intracel ular adenosine triphosphatei value showed that about 71.4% (n=25) patients in the infection group distributed in the low immune response zone, and the percentage in the infection group was significantly higher than that in the other three groups (P < 0.05). The intracel ular adenosine triphosphatei value in the infection group was significantly lower than that in the other three groups. That indicated that the infection was correlated with low cel ular mediated immune function, and the detection of immune cel function played an important role in monitoring the infection after renal transplantation. The immune cel function monitoring with ImmuKnowTM-Cylex? assay provides reliable and objective basis for the diagnosis and treatment of infection after renal transplantation, but it has no correlation with the rejection after renal transplantation, and that needs further investigation with a large-sample experiment.