CAJ | 학술논문

背景:在肝脏移植过程中,低温保存和缺血可导致肝脏产生氧自由基而损伤肝组织.目的:研究自制器官保存液对低温保存大鼠肝脏氧自由基的影响,并与器官保存液的“金标准”UW 液进行对比.方法:16只9周龄 SD 大鼠随机分为实验组和对照组,每组8只.建立 SD 大鼠肝脏灌注模型,分别用自制器官保存液组和 UW 液组灌洗肝脏,取出肝脏置于4℃保存液中,于保存24,48,72 h 后分别检测肝脏超氧化物歧化酶、一氧化氮合酶、总抗氧化能力和活性丙二醛水平.结果与结论:自制器官保存液对大鼠肝脏低温保存各时点超氧化物歧化酶、一氧化氮合酶、总抗氧化能力活性及丙二醛含量与 UW 液组比较差异均无显著性意义(P >0.05),表明自制器官保存液能够减轻缺血再灌注后氧自由基对大鼠肝脏的损伤.自制器官保存液对低温保存大鼠肝脏在减轻氧自由基损伤方面有较好的效果,与 UW 液相当.
배경:재간장이식과정중,저온보존화결혈가도치간장산생양자유기이손상간조직.목적:연구자제기관보존액대저온보존대서간장양자유기적영향,병여기관보존액적“금표준”UW 액진행대비.방법:16지9주령 SD 대서수궤분위실험조화대조조,매조8지.건립 SD 대서간장관주모형,분별용자제기관보존액조화 UW 액조관세간장,취출간장치우4℃보존액중,우보존24,48,72 h 후분별검측간장초양화물기화매、일양화담합매、총항양화능력화활성병이철수평.결과여결론:자제기관보존액대대서간장저온보존각시점초양화물기화매、일양화담합매、총항양화능력활성급병이철함량여 UW 액조비교차이균무현저성의의(P >0.05),표명자제기관보존액능구감경결혈재관주후양자유기대대서간장적손상.자제기관보존액대저온보존대서간장재감경양자유기손상방면유교호적효과,여 UW 액상당.
@@@@BACKGROUND: During liver transplantation, cryopreservation and ischemia can lead to production of oxygen free radicals by the liver which damages liver tissue. OBJECTIVE: To study the effects of self-made cryopreservation solution on oxygen free radicals in cryopreserved rat liver, and to compare with “gold standard” organ preservation solution-UW solution.METHODS: Sixteen 9-week-old Sprague Dawley rats were randomly divided into two groups: experimental group and control group, 8 rats in each group. Sprague Dawley rat liver perfusion models were established, and the livers in each group were lavaged with self-made organ preservation solution and UW preservation solution, then removed the liver and placed into 4℃preservation solution. The superoxide dismutase, nitric oxide synthase, total antioxidant capacity activity and the malondialdehyde content were detected at 24, 48 and 72 hours after preservation. RESULTS AND CONCLUSION: There were no significant differences in superoxide dismutase, nitric oxide synthase, total antioxidant capacity activity and the malondialdehyde content in rat liver tissue cryopreserved in the self-made organ preservation solution versus UW solution (P > 0.05). These findings suggest that self-made organ preservation solution can reduce the damage of oxygen free radical to the livers of rats after ischemia-reperfusion and the effect is equivalent to UW solution.