中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
5期
859-865
,共7页
李川%戚峰%刘彤%李富新%王鹏志
李川%慼峰%劉彤%李富新%王鵬誌
리천%척봉%류동%리부신%왕붕지
器官移植%心肺移植%异种心脏移植%小鼠%大鼠%动物模型%异位%袖套法%排斥反应%国家自然科学基金%器官移植图片文章
器官移植%心肺移植%異種心髒移植%小鼠%大鼠%動物模型%異位%袖套法%排斥反應%國傢自然科學基金%器官移植圖片文章
기관이식%심폐이식%이충심장이식%소서%대서%동물모형%이위%수투법%배척반응%국가자연과학기금%기관이식도편문장
organ transplantation%heart-lung transplantation%cardiac xenotransplantation%mice%rats%animal models%ectopic%cuff method%rejection%the National Natural Science Foundation of China%photographs-containing paper of organ transplantation
背景:小动物心脏移植模型是器官移植基础与临床研究的常用模型和重要手段,移植部位多为颈部和腹部.其中颈部异位移植最大的优点在于移植心位于颈部皮下,有利于对供心搏动情况进行直接观察,进而早期预判排斥反应的发生.目的:对小鼠-大鼠颈部异种心脏移植模型的麻醉方式、手术操作、围手术期处理等方面进行改进,以期建立更加稳定的动物模型.方法:采用改良袖套法建立异位异种心脏移植模型,将小鼠供心移植到受体大鼠的右侧颈部,主要改进了摘取供心的方法,并使用小动物麻醉机进行异氟烷吸入性麻醉.模型建立分为练习、稳定和定型3个阶段.取部分实验标本做组织病理学检查.结果与结论:模型建立练习阶段、稳定阶段和定型阶段手术成功率分别为53.33%,85.71%和96.15%稳定及定型阶段手术成功率明显高于练习阶段(P <0.05);稳定和定型阶段手术总操作时间明显短于练习阶段(P <0.05).组织病理学结果显示,异种心脏移植后出现供心血管内皮损伤、血栓形成、心肌实质损伤、间质出血和炎症细胞浸润等现象,较正常心脏和同系移植均有明显改变,并且有随移植术后时间延长而逐渐加重的趋势,证实了模型的可靠性.提示小鼠-大鼠颈部异种心脏移植模型操作简便、稳定可靠,短时间内即可熟练掌握,成功率高,是研究异种移植排斥反应的理想动物模型.
揹景:小動物心髒移植模型是器官移植基礎與臨床研究的常用模型和重要手段,移植部位多為頸部和腹部.其中頸部異位移植最大的優點在于移植心位于頸部皮下,有利于對供心搏動情況進行直接觀察,進而早期預判排斥反應的髮生.目的:對小鼠-大鼠頸部異種心髒移植模型的痳醉方式、手術操作、圍手術期處理等方麵進行改進,以期建立更加穩定的動物模型.方法:採用改良袖套法建立異位異種心髒移植模型,將小鼠供心移植到受體大鼠的右側頸部,主要改進瞭摘取供心的方法,併使用小動物痳醉機進行異氟烷吸入性痳醉.模型建立分為練習、穩定和定型3箇階段.取部分實驗標本做組織病理學檢查.結果與結論:模型建立練習階段、穩定階段和定型階段手術成功率分彆為53.33%,85.71%和96.15%穩定及定型階段手術成功率明顯高于練習階段(P <0.05);穩定和定型階段手術總操作時間明顯短于練習階段(P <0.05).組織病理學結果顯示,異種心髒移植後齣現供心血管內皮損傷、血栓形成、心肌實質損傷、間質齣血和炎癥細胞浸潤等現象,較正常心髒和同繫移植均有明顯改變,併且有隨移植術後時間延長而逐漸加重的趨勢,證實瞭模型的可靠性.提示小鼠-大鼠頸部異種心髒移植模型操作簡便、穩定可靠,短時間內即可熟練掌握,成功率高,是研究異種移植排斥反應的理想動物模型.
배경:소동물심장이식모형시기관이식기출여림상연구적상용모형화중요수단,이식부위다위경부화복부.기중경부이위이식최대적우점재우이식심위우경부피하,유리우대공심박동정황진행직접관찰,진이조기예판배척반응적발생.목적:대소서-대서경부이충심장이식모형적마취방식、수술조작、위수술기처리등방면진행개진,이기건립경가은정적동물모형.방법:채용개량수투법건립이위이충심장이식모형,장소서공심이식도수체대서적우측경부,주요개진료적취공심적방법,병사용소동물마취궤진행이불완흡입성마취.모형건립분위연습、은정화정형3개계단.취부분실험표본주조직병이학검사.결과여결론:모형건립연습계단、은정계단화정형계단수술성공솔분별위53.33%,85.71%화96.15%은정급정형계단수술성공솔명현고우연습계단(P <0.05);은정화정형계단수술총조작시간명현단우연습계단(P <0.05).조직병이학결과현시,이충심장이식후출현공심혈관내피손상、혈전형성、심기실질손상、간질출혈화염증세포침윤등현상,교정상심장화동계이식균유명현개변,병차유수이식술후시간연장이축점가중적추세,증실료모형적가고성.제시소서-대서경부이충심장이식모형조작간편、은정가고,단시간내즉가숙련장악,성공솔고,시연구이충이식배척반응적이상동물모형.
BACKGROUND: Smal animal heart transplantation model is a common model and an important mean for basic and clinical research of organ transplantation, and the transplant part is often the neck and abdomen. The biggest advantage of cervical heterotopic transplantation is that the transplanted heart wil located in the neck subcutaneous, which is conducive to the direct observation of the beating of donor heart, thus early anticipating the rejection. OBJECTIVE: To improve the anesthesia, surgical procedures and perioperative management of the mouse-rat cervical cardiac xenotransplantation model, in order to establish a more stable animal model. METHODS: The mouse-rat heterotopic cardiac xenotransplantation models were established by modified cuff technique to transfer the donor heart of mouse to the right side of the neck of rat, the method to remove the donor heart was improved, and the models received inhalation anesthesia of isoflurane with smal animal anesthesia machine. The experimental levels of establishing the models were divided into practicing, stable and skil ed stages. Part samples were taken for the histopathological examination. RESULTS AND CONCLUSION: In the three stages (practicing, stable, skil ed) of establishing the models, the successful rates of surgery were 53.33%, 85.71%, and 96.15% respectively. The successful rate in stable and skil ed stages was higher than that in the practicing stage (P < 0.05). The total operation time in the stable and skil ed stages was significantly shorter than that in the practicing stage (P < 0.05). Histopathological examination results showed that the cardiovascular endothelial injury, thrombosis, myocardial parenchymal damage, interstitial hemorrhage and inflammatory cel infiltration after heterotopic cardiac xenotransplantation were more serious compared with normal heart transplantation and cardiac al ograft, and showed a time-depend manner, which identify the reliability of the model. It suggests that the mouse-rat cervical cardiac xenotransplantation model is simple, reliable, easily operated in a short-time and has a high successful rate. It is an ideal animal model for studying xenotransplantation rejection.
