CAJ | 학술논문

背景:脂肪干细胞具有多向分化潜能,在组织工程和再生医学领域具有广阔的应用前景.目的:随访评估脂肪干细胞在自体脂肪移植隆胸中的技术要点及治疗效果.方法:随访15例自体脂肪颗粒注射隆胸患者3-6个月.脂肪移植隆胸过程分为两期,一期用于脂肪干细胞的分离与扩增,抽出脂肪量为10-20 mL.用胶原酶Ⅰ消化,贴壁培养获得脂肪干细胞,扩增至第2代达到1×108,并进行细胞免疫表型,分化及遗传稳定性鉴定.二期抽取脂肪量300-450 mL,脂肪混合脂肪干细胞用于移植填充.结果与结论:第2代自体脂肪干细胞具有典型的间充质干细胞的特征,移植后3-6个月回访无明显脂肪吸收现象,无感染、疼痛、上肢活动受限等不良反应,隆胸效果良好.作者认为自体脂肪干细胞介导的自体脂肪注射隆胸是一种切实可行有效的方法.
배경:지방간세포구유다향분화잠능,재조직공정화재생의학영역구유엄활적응용전경.목적:수방평고지방간세포재자체지방이식륭흉중적기술요점급치료효과.방법:수방15례자체지방과립주사륭흉환자3-6개월.지방이식륭흉과정분위량기,일기용우지방간세포적분리여확증,추출지방량위10-20 mL.용효원매Ⅰ소화,첩벽배양획득지방간세포,확증지제2대체도1×108,병진행세포면역표형,분화급유전은정성감정.이기추취지방량300-450 mL,지방혼합지방간세포용우이식전충.결과여결론:제2대자체지방간세포구유전형적간충질간세포적특정,이식후3-6개월회방무명현지방흡수현상,무감염、동통、상지활동수한등불량반응,륭흉효과량호.작자인위자체지방간세포개도적자체지방주사륭흉시일충절실가행유효적방법.
@@@@BACKGROUND: Human adipose stem cells have the capability of multipotential differentiation and have a promising prospect in the field of tissue engineering and regenerative medicine. OBJECTIVE: To evaluate the technology and efficacy of human adipose stem cells in autologous fat grafting breast augmentation. METHODS: Fifteen patients with auto-adipose injection breast augmentation were fol owed-up for 3-6 months. The whole operation was separated into two phases, in the first phase, 15-20 mL adipose tissue was aspirated and digested with col agenase Ⅰ to separate and amplify the human adipose stem cells. When cultured to passage 2, the plastic adherent stem cells were up to 1×108 and verificated by immunophenotype, differentiation and genetic stability assays before transplantation. In the second phase, 300-450 mL adipose tissue was aspirated and transplanted combined with human adipose stem cells. RESULTS AND CONCLUSION: The passage 2 human adipose stem cells exhibited typical mesenchymal stem cells characteristics and maintained genetic stability. The 3-6 months fol ow-up demonstrated that there was no apparent adipose absorption, infection, pain and upper limb activity limitation, and the effect was good. The authors suggested that autologous adipose derived stem cells-mediated auto-adipose injection breast augmentation is a practical and efficacious method.