CAJ | 학술논문

干细胞%骨髓干细胞%骨髓间充质干细胞%β射线%皮肤损伤%创面%创面愈合%大鼠%成纤维细胞%组织再生%组织工程%其他基金%干细胞图片文章榦細胞%骨髓榦細胞%骨髓間充質榦細胞%β射線%皮膚損傷%創麵%創麵愈閤%大鼠%成纖維細胞%組織再生%組織工程%其他基金%榦細胞圖片文章
간세포%골수간세포%골수간충질간세포%β사선%피부손상%창면%창면유합%대서%성섬유세포%조직재생%조직공정%기타기금%간세포도편문장
stem cells%bone marrow-derived stem cells%bone marrow mesenchymal stem cells%β ray%skin injury%wound%wound healing%rats%fibroblasts%tissue regeneration%tissue engineering%other grants-supported paper%stem cel photographs-containing paper

背景:β射线皮肤损伤创面难愈,尚无有效的治疗方法.目的:观察骨髓间充质干细胞对β射线皮肤损伤创面愈合的影响.方法:①选用 SD 雌性大鼠3只分离培养骨髓间充质干细胞,传代细胞生长至第5代,DAPI 标记细胞,制备骨髓间充质干细胞悬液.②选3月龄清洁级 SD 雌性大鼠47只,随机分为3组:治疗组应用直线加速器产生的β射线(45 Gy)单次照射大鼠臀部皮肤40 mm×30 mm,建立急性深Ⅱ度β射线皮肤损伤动物模型,创面出现后将骨髓间充质干细胞悬液注入大鼠创面皮下及真皮层;对照组β射线照射同治疗组,创面出现后注射安慰剂,用法同治疗组.正常组为正常大鼠.光镜下观察治疗后1,2,3,4周各组大鼠创面组织病理学变化,免疫组织化学方法检测 CD31、CK4、成纤维细胞生长因子含量的动态变化.结果与结论:治疗组创面愈合时间比对照组明显加快(P <0.05).治疗组注射骨髓间充质干细胞细胞悬液后1-4周 CD31、CK4、成纤维细胞生长因子阳性细胞值明显高于对照组(P <0.05).证实骨髓间充质干细胞能促进β射线皮肤损伤创面的愈合,缩短创面愈合的时间.
배경:β사선피부손상창면난유,상무유효적치료방법.목적:관찰골수간충질간세포대β사선피부손상창면유합적영향.방법:①선용 SD 자성대서3지분리배양골수간충질간세포,전대세포생장지제5대,DAPI 표기세포,제비골수간충질간세포현액.②선3월령청길급 SD 자성대서47지,수궤분위3조:치료조응용직선가속기산생적β사선(45 Gy)단차조사대서둔부피부40 mm×30 mm,건립급성심Ⅱ도β사선피부손상동물모형,창면출현후장골수간충질간세포현액주입대서창면피하급진피층;대조조β사선조사동치료조,창면출현후주사안위제,용법동치료조.정상조위정상대서.광경하관찰치료후1,2,3,4주각조대서창면조직병이학변화,면역조직화학방법검측 CD31、CK4、성섬유세포생장인자함량적동태변화.결과여결론:치료조창면유합시간비대조조명현가쾌(P <0.05).치료조주사골수간충질간세포세포현액후1-4주 CD31、CK4、성섬유세포생장인자양성세포치명현고우대조조(P <0.05).증실골수간충질간세포능촉진β사선피부손상창면적유합,축단창면유합적시간.
@@@@BACKGROUND: The β-ray-irradiated skin injury is difficult to heal and there is no effective treatment method for β-ray-irradiated skin injury. OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cel transplantation in treatment of β-ray-irradiated skin injury in rats. METHODS: Three Sprague-Dawley female rats were kil ed to isolate and culture the bone marrow mesenchymal stem cells, then the cells were labeled with 4',6-diamidino-2-phenylindole to prepare the @@@@bone marrow mesenchymal stem cells suspension after passaged to the fifth generation. Forty-seven Sprague-Dawley female rats of 3 months old and clean grade were randomly divided into three groups: treatment group, control group, and normal group. In the treatment group, single dosage (45 Gy) of β-ray irradiation produced by linear accelerator was applied on buttock skin (40 mm×30 mm) in rats, and the acute deep injury model was established. Bone marrow mesenchymal stem cellsuspension was injected in the subcutaneous and dermal layers after the wound appeared. The rats in the control group were irradiated as those in the treatment group, and placebo was injected when the wound appeared, with the same method as the treatment group. Rats in the normal group were not irradiated. The pathological changes of wound tissue of rats in each group were observed at 1, 2, 3 and 4 weeks after treatment under light microscope, and the concentration of CD31, CK4 and fibroblast growth factor was detected by immunohistochemistry. RESULTS AND CONCLUSION: The wound healing time in the treatment group was shorter than that in the control group (P < 0.05). Immunohistochemistry showed the number of CD31, CK4 and fibroblast growth factor positive cells in the treatment group at 1-4 weeks after bone mesenchymal stem cellsuspension injection was significantly greater than that in the control group (P < 0.05). It indicates that bone mesenchymal stem cel injection can promote the wound healing of β-ray-irradiated skin injury and reduce wound healing time.