CAJ | 학술논문

背景:目前临床应用的人工瓣膜,无论机械瓣还是生物瓣都存在着诸如易感染、出血和血栓形成并发症,因瓣口狭窄需再手术等自身难以克服的缺陷.理论上具有生物活性的组织工程心脏瓣膜可克服上述缺点,但种子细胞和瓣膜支架的选取尚存在争议.目的:探讨应用人骨髓基质干细胞及脱细胞猪主动脉瓣支架体外构建组织工程瓣膜的可行性.方法:采用去垢剂-核酸酶消化法处理,去除猪主动脉瓣叶细胞成分,获取施行简单先心病修补患者胸骨来源的骨髓基质干细胞,将其种植于脱细胞猪主动脉瓣支架共培养5 d.结果与结论:流式细胞技术证实接种的种子细胞的表面抗原符合人骨髓基质干细胞的特征;光镜及电镜检查证实,猪主动脉瓣膜中的细胞成分可完全去除,获得完整无细胞的纤维网状支架;瓣叶去细胞前后的生物力学性能无明显变化;种植的人骨髓基质干细胞可在脱细胞猪主动脉瓣支架表面形成一层连续的细胞层;接种的人骨髓基质干细胞有向成纤维细胞分化的趋势.以上结果提示种植人骨髓基质干细胞于脱细胞猪主动脉瓣支架上,可构建出组织工程心脏瓣膜.
배경:목전림상응용적인공판막,무론궤계판환시생물판도존재착제여역감염、출혈화혈전형성병발증,인판구협착수재수술등자신난이극복적결함.이론상구유생물활성적조직공정심장판막가극복상술결점,단충자세포화판막지가적선취상존재쟁의.목적:탐토응용인골수기질간세포급탈세포저주동맥판지가체외구건조직공정판막적가행성.방법:채용거구제-핵산매소화법처리,거제저주동맥판협세포성분,획취시행간단선심병수보환자흉골래원적골수기질간세포,장기충식우탈세포저주동맥판지가공배양5 d.결과여결론:류식세포기술증실접충적충자세포적표면항원부합인골수기질간세포적특정;광경급전경검사증실,저주동맥판막중적세포성분가완전거제,획득완정무세포적섬유망상지가;판협거세포전후적생물역학성능무명현변화;충식적인골수기질간세포가재탈세포저주동맥판지가표면형성일층련속적세포층;접충적인골수기질간세포유향성섬유세포분화적추세.이상결과제시충식인골수기질간세포우탈세포저주동맥판지가상,가구건출조직공정심장판막.
BACKGROUND: Nowadays, mechanical or biological valve recipients used in the clinic are stil at the risk of infection, hemorrhage, thrombosis and reoperation owing to valve stenosis. Tissue-engineered heart valve with biological activity can overcome the disadvantages above. While, the optimal choice of scaffolds and seeding cells remains disputable. OBJECTIVE: To explore the feasibility to construct tissue-engineered heart valve with acel ularized porcine aortic valve scaffold and human bone marrow stromal stem cells in vitro. METHODS: The porcine aortic valves were decel ularized with the detergent and enzymatic extraction process to remove the cel ular components. Human bone marrow stromal stem cells were aspirated from sternum of the patients with simple congenital heart malformation, and then the cells were seeded on the acel ularized porcine aortic valve scaffold and cultured for 5 days. RESULTS AND CONCLUSION: Flow cytometry identified that the characteristics of surface antigen of the inoculated seed cells were in line with those of human bone marrow stromal stem cells. Light microscopy and electron microscopy confirmed that the cel ular components in the porcine aortic valves could be removed to obtain the complete acel ular fiber mesh stent. There was no significant difference in biomechanical property between before and after acel ularization. The human bone marrow stromal stem cells implanted on the acel ularized porcine aortic valve scaffold could form a continuous cel layer on the surfaces of the scaffold. The inoculated bone marrow stromal stem cells could be differentiated into fibroblasts. The implantation of human bone marrow stromal stem cells on the acel ularized porcine aortic valve scaffold can construct the tissue-engineered heart valve.