CAJ | 학술논문

背景:旋转生物反应器是目前应用较多的微载体培养系统.目的:应用微载体旋转立体培养方法对成人退变髓核细胞进行扩增及鉴定.方法:对手术切除的髓核组织进行原代培养,传代后进行单层培养和微载体旋转立体培养的对比,并行苏木精-伊红、Gimsa 染色观察细胞形态,利用电镜观察其超微结构,利用 MTT 比色法检测细胞生长活性.结果与结论:倒置相差显微镜观察培养的细胞符合退变髓核细胞形态,透射电镜下可显示其退变改变.倒置相差显微镜和扫描电镜下观察,可见退变的髓核细胞呈立体状生长,并能够成功甩珠传代.细胞在对数生长期时,微载体培养的细胞的生长活性明显高于单层培养组的细胞.表明微载体旋转立体培养法可以较好的维持细胞表型,对对数生长期髓核细胞的细胞活性具有明显的刺激作用.
배경:선전생물반응기시목전응용교다적미재체배양계통.목적:응용미재체선전입체배양방법대성인퇴변수핵세포진행확증급감정.방법:대수술절제적수핵조직진행원대배양,전대후진행단층배양화미재체선전입체배양적대비,병행소목정-이홍、Gimsa 염색관찰세포형태,이용전경관찰기초미결구,이용 MTT 비색법검측세포생장활성.결과여결론:도치상차현미경관찰배양적세포부합퇴변수핵세포형태,투사전경하가현시기퇴변개변.도치상차현미경화소묘전경하관찰,가견퇴변적수핵세포정입체상생장,병능구성공솔주전대.세포재대수생장기시,미재체배양적세포적생장활성명현고우단층배양조적세포.표명미재체선전입체배양법가이교호적유지세포표형,대대수생장기수핵세포적세포활성구유명현적자격작용.
@@@@BACKGROUND: Rotating bioreactor is a microcarrier-based stirred culture system that is currently used more often. OBJECTIVE: To culture the adult degenerative nucleus pulposus cells in vitro by a microcarrier-based stirred culture system, and then to amplify and identify the cells. METHODS: Nucleus pulposus specimens taken from patients with disc degenerative diseases were randomly divided into monolayer culture group and micro-carrier culture group. The cel morphology was observed by hematoxylin-eosin staining and Gimsa staining, the microstructure was observed under an electron microscope, cel viability was detected by MTT method. RESULTS AND CONCLUSION: The morphology of degenerative nucleus pulpous cells was identified by inverted phase contrast microscope, and the regression was showed by a transmission electron microscope. The microcarrier cultured cells were observed by an inverted phase contrast microscope and scanning electron microscope showing stereoscopic shape. In the logarithmic growth phase, the growth activity of microcarrier culture cells was significantly higher than that of the cells in monolayer culture. Microcarrier-based stirred culture system can stabilize the cel phenotype, and significantly stimulate the cel ular activity of nucleus pulposus cells in the logarithmic growth phase.