CAJ | 학술논문

背景:近年研究发现,激素性股骨头坏死模型中存在大量凋亡骨细胞,而 Caspase-3及 Bcl-2为调节细胞凋亡的两个重要因子.目的:观察 Bcl-2和 Caspase-3在激素性股骨头坏死大鼠模型中的表达及二者的相关性.方法:选取健康 Wistar 大鼠60只随机分为2组,实验组臀肌注射泼尼松龙12.5 mg/kg,每周2次,制备激素性股骨头坏死模型;对照组注射等量生理盐水.分别于给药后4,8,12周获取离体大鼠股骨头,采用苏木精-伊红染色观察骨组织形态学改变,免疫组织化学染色观察 Bcl-2和 Caspase-3蛋白的表达水平,并采用直线相关分析 Bcl-2和 Caspase-3的相关性.结果与结论:对照组及实验组股骨头外形均完整,软骨面平整、光滑.随着给药时间的延长,实验组大鼠股骨头内骨小梁逐渐变细,脂肪细胞增多,骨陷窝空虚率明显升高.免疫组织化学染色显示:与对照组比较,实验组大鼠股骨头 Caspase-3表达明显增高(P <0.01),且随给药时间的延长呈逐渐增高趋势;而 Bcl-2的表达显著低于对照组(P <0.01).相关分析结果显示 Bcl-2和 Caspase-3在各组内的表达不具有相关性.提示激素可提高 Caspase-3的表达促进骨细胞凋亡,降低 Bcl-2的表达抑制抗凋亡作用,进而导致激素性股骨头坏死的发生和发展.
배경:근년연구발현,격소성고골두배사모형중존재대량조망골세포,이 Caspase-3급 Bcl-2위조절세포조망적량개중요인자.목적:관찰 Bcl-2화 Caspase-3재격소성고골두배사대서모형중적표체급이자적상관성.방법:선취건강 Wistar 대서60지수궤분위2조,실험조둔기주사발니송룡12.5 mg/kg,매주2차,제비격소성고골두배사모형;대조조주사등량생리염수.분별우급약후4,8,12주획취리체대서고골두,채용소목정-이홍염색관찰골조직형태학개변,면역조직화학염색관찰 Bcl-2화 Caspase-3단백적표체수평,병채용직선상관분석 Bcl-2화 Caspase-3적상관성.결과여결론:대조조급실험조고골두외형균완정,연골면평정、광활.수착급약시간적연장,실험조대서고골두내골소량축점변세,지방세포증다,골함와공허솔명현승고.면역조직화학염색현시:여대조조비교,실험조대서고골두 Caspase-3표체명현증고(P <0.01),차수급약시간적연장정축점증고추세;이 Bcl-2적표체현저저우대조조(P <0.01).상관분석결과현시 Bcl-2화 Caspase-3재각조내적표체불구유상관성.제시격소가제고 Caspase-3적표체촉진골세포조망,강저 Bcl-2적표체억제항조망작용,진이도치격소성고골두배사적발생화발전.
BACKGROUND: Recent studies have shown that a large amount of apoptotic osteocytes in the model of steroid-induced necrosis of the femoral head. Caspase-3 and Bcl-2 are two important factors for regulation of cel apoptosis. OBJECTIVE: To observe Bcl-2 and Caspase-3 levels in a rat model of femoral head necrosis and their correlation. METHODS: Sixty healthy Wistar rats were randomly divided into two groups (n=30). Experimental group was treated with gluteal muscle injection of prednisolone 12.5 mg/kg, twice per week, for establishing a femoral head necrosis model; control group was injected with the same amount of normal saline. Rat femoral head was removed after 4, 8 and 12 weeks respectively. Bone tissue morphological changes were observed by hematoxylin-eosin staining and the protein expression levels of Bcl-2 and Caspase-3 were observed using immunohistochemical staining. Linear correlation analysis was performed for analysis of correlation between Bcl-2 and Caspase-3. RESULTS AND CONCLUSION: Femoral heads were complete in the experimental and control groups with smooth and flat cartilage surface. In the experimental group, the trabecular bone in the femoral head gradual y became thinner, the number of adipose cells increased and percentage of empty lacunae significantly increased with the time of administration. Caspase-3 expression increased significantly in the experimental group compared with the control group (P < 0.01), trending to gradual y increase with the time of administration. Conversely, the expression of Bcl-2 was lower in the experimental group than the control group (P < 0.01). The linear correlation analysis showed that there was no correlation between Bcl-2 and Caspase-3. These findings indicate that steroids can increase the expression of Caspase-3 promoting apoptosis of osteocytes and reduce the expression of Bcl-2 inhibiting anti-apoptotic role, which leads to the occurrence and development of steroid-induced necrosis of the femoral head.