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组织构建%组织构建与生物力学%力学应变%破骨细胞%凋亡%线粒体%骨重建%体外培养%生理强度载荷%病理强度载荷%线粒体膜电位%破骨细胞分化因子%国家自然科学基金%组织构建图片文章組織構建%組織構建與生物力學%力學應變%破骨細胞%凋亡%線粒體%骨重建%體外培養%生理彊度載荷%病理彊度載荷%線粒體膜電位%破骨細胞分化因子%國傢自然科學基金%組織構建圖片文章
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背景:力学应变在骨重建中起重要作用.然而,力学应变是否影响破骨细胞的凋亡仍不清楚.目的:观察力学应变对体外培养破骨细胞凋亡的影响.方法:对小鼠单核细胞 RAW264.7采用巨噬细胞集落刺激因子和破骨细胞分化因子诱导,抗酒石酸酸性磷酸酶染色和骨吸收实验确定成功诱导出了破骨细胞.实验共分为3组,对诱导的破骨细胞分别施加0(对照组),2500和5000με的基底拉伸应变3 d,1 h/次,1次/d.结果与结论:与对照组相比,生理强度载荷2500με阻止了破骨细胞的凋亡和线粒体膜电位的下降.但是,病理强度载荷5000με对破骨细胞的凋亡和线粒体膜电位没有影响.
배경:역학응변재골중건중기중요작용.연이,역학응변시부영향파골세포적조망잉불청초.목적:관찰역학응변대체외배양파골세포조망적영향.방법:대소서단핵세포 RAW264.7채용거서세포집락자격인자화파골세포분화인자유도,항주석산산성린산매염색화골흡수실험학정성공유도출료파골세포.실험공분위3조,대유도적파골세포분별시가0(대조조),2500화5000με적기저랍신응변3 d,1 h/차,1차/d.결과여결론:여대조조상비,생리강도재하2500με조지료파골세포적조망화선립체막전위적하강.단시,병리강도재하5000με대파골세포적조망화선립체막전위몰유영향.
BACKGROUND: Mechanical strain plays an important role in bone remodeling. However, it remains unclear that whether mechanical strain has an influence on osteoclast apoptosis. OBJECTIVE: To investigate the effects of mechanical strain on osteoclast apoptosis in vitro. METHODS: RAW264.7 cells were treated with macrophage colony-stimulating factor and receptor activator of nuclear factor kappa-B ligand. Then, tartrate-resistant acid phosphatase staining and resorption assay were used to confirm that whether mature osteoclasts were induced successful y. Then the induced osteoclasts were subjected to 0, 2 500 and 5 000 με mechanical stretch strain for 3 days, once for 1 hour per day. RESULTS AND CONCLUSION: Compared to the control group, 2 500 με mechanical stretch strain inhibited osteoclast apoptosis and the col apse of mitochondrial membrane potential. But, the cells subjected to 5 000 με mechanical stretch strain showed no significant differences compared to the control group.