CAJ | 학술논문

背景:自体髂骨移植一直被认为是骨缺损修复的“金标准”,但其来源有限.目的:验证应用可注射骨修复材料结合骨碎补总黄酮修复大鼠颅骨缺损的效果.方法:80只雄性 SD 大鼠建立双侧颅骨缺损模型,随机分为3组:骨修复材料+骨碎补总黄酮组采用可注射骨修复材料结合骨碎补总黄酮灌胃修复大鼠颅骨缺损;骨修复材料+去离子水组采用可注射骨修复材料结合去离子水灌胃修复大鼠颅骨缺损;羟基磷灰石+去离子水组采用羟基磷灰石结合去离子水灌胃修复大鼠颅骨缺损,1次/d,持续8周.于建模后2,4,8周取颅骨标本进行苏木精-伊红染色和 Masson染色组织学观察.结果与结论:羟基磷灰石组新骨形成和材料降解速度较慢;可注射骨修复材料组新骨形成和材料降解较羟基磷灰石组快,利于血管及纤维组织长入;骨碎补总黄酮灌胃可以促进血管及纤维组织长入材料,促进成骨.与羟基磷灰石相比,可注射骨修复材料结合骨碎补总黄酮修复大鼠颅骨缺损,可促进新骨形成,缩短骨缺损修复时间.
배경:자체가골이식일직피인위시골결손수복적“금표준”,단기래원유한.목적:험증응용가주사골수복재료결합골쇄보총황동수복대서로골결손적효과.방법:80지웅성 SD 대서건립쌍측로골결손모형,수궤분위3조:골수복재료+골쇄보총황동조채용가주사골수복재료결합골쇄보총황동관위수복대서로골결손;골수복재료+거리자수조채용가주사골수복재료결합거리자수관위수복대서로골결손;간기린회석+거리자수조채용간기린회석결합거리자수관위수복대서로골결손,1차/d,지속8주.우건모후2,4,8주취로골표본진행소목정-이홍염색화 Masson염색조직학관찰.결과여결론:간기린회석조신골형성화재료강해속도교만;가주사골수복재료조신골형성화재료강해교간기린회석조쾌,리우혈관급섬유조직장입;골쇄보총황동관위가이촉진혈관급섬유조직장입재료,촉진성골.여간기린회석상비,가주사골수복재료결합골쇄보총황동수복대서로골결손,가촉진신골형성,축단골결손수복시간.
@@@@BACKGROUND: Autogenous iliac bone graft has been considered as the “gold standard” for repair of bone defects, but its resource is limited. OBJECTIVE: To analyze the effect of injectable bone regeneration composite (IBRC) combined withassemble flavone of rhizome drynaria (AFDR) in the repair of rat skul defects. METHODS: Eighty male Sprague-Dawley rats were randomly arranged into three groups, and rats in each group were intragastrical y administered with IBRC+AFDR, IBRC+deionized water, and hydroxyapatite+deionized water, respectively. Treatment was performed once a day, lasting for 8 weeks. Skul samples were taken for hematoxylin-eosin and Masson staining at 2, 4, and 8 weeks after modeling. RESULTS AND CONCLUSION: New bone formation and material degradation were slower in the hydroxyapatite+deionized water group than the IBRC+AFDR and IBRC+deionized water groups. IBRC was beneficial for the in-growth of vessels and fibers. Intragastric administration of AFDR could induce bone formation through improving the growth of vessels and fibers into the implant. IBRC+AFDR can promote bone formation and shorten repair time during the repair of rat skul defects.