CAJ | 학술논문

背景:与传统的热疗方法相比,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒可以同时发挥 As2O3的细胞毒性作用和磁感应加热的联合定向治疗作用,效果优于单一治疗.目的:制备 As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒,观察其对食管癌 Eca109细胞增殖的抑制作用.方法:采用浸渍法制备 As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒,含砷量0.012%,以透射电镜、能谱仪、原子分光光度仪对其进行表征.向两块培养板的食管癌 Eca109细胞中分别加入 DMEM 培养液(阴性对照)、Mn0.5Zn0.5Fe2O4纳米材料、含 As2O3终浓度5μmol/L 的 As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒、游离As2O3(终浓度5μmol/L),其中一块培养板进行磁流体热疗,另一块培养板正常培养.结果与结论:As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒近似球形,As2O3成功浸渍在 Mn0.5Zn0.5Fe2O4纳米材料表面,砷含量在0.012%-0.066%之间.当 As2O3浓度为5μmol/L 时,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒组细胞增殖率明显低于阴性对照组和 Mn0.5Zn0.5Fe2O4纳米材料组(P <0.05);而在磁流体热疗中, As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒组细胞增殖率明显低于游离 As2O3组或 Mn0.5Zn0.5Fe2O4组(P <0.05);在凋亡率检测中,As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒联合磁流体热疗组细胞凋亡率明显高于Mn0.5Zn0.5Fe2O4纳米材料联合磁流体热疗组或游离 As2O3组(P <0.05).表明 As2O3/Mn0.5Zn0.5Fe2O4复合纳米粒联合磁流体热疗可显著抑制食管癌细胞增殖.
배경:여전통적열료방법상비,As2O3/Mn0.5Zn0.5Fe2O4복합납미립가이동시발휘 As2O3적세포독성작용화자감응가열적연합정향치료작용,효과우우단일치료.목적:제비 As2O3/Mn0.5Zn0.5Fe2O4복합납미립,관찰기대식관암 Eca109세포증식적억제작용.방법:채용침지법제비 As2O3/Mn0.5Zn0.5Fe2O4복합납미립,함신량0.012%,이투사전경、능보의、원자분광광도의대기진행표정.향량괴배양판적식관암 Eca109세포중분별가입 DMEM 배양액(음성대조)、Mn0.5Zn0.5Fe2O4납미재료、함 As2O3종농도5μmol/L 적 As2O3/Mn0.5Zn0.5Fe2O4복합납미립、유리As2O3(종농도5μmol/L),기중일괴배양판진행자류체열료,령일괴배양판정상배양.결과여결론:As2O3/Mn0.5Zn0.5Fe2O4복합납미립근사구형,As2O3성공침지재 Mn0.5Zn0.5Fe2O4납미재료표면,신함량재0.012%-0.066%지간.당 As2O3농도위5μmol/L 시,As2O3/Mn0.5Zn0.5Fe2O4복합납미립조세포증식솔명현저우음성대조조화 Mn0.5Zn0.5Fe2O4납미재료조(P <0.05);이재자류체열료중, As2O3/Mn0.5Zn0.5Fe2O4복합납미립조세포증식솔명현저우유리 As2O3조혹 Mn0.5Zn0.5Fe2O4조(P <0.05);재조망솔검측중,As2O3/Mn0.5Zn0.5Fe2O4복합납미립연합자류체열료조세포조망솔명현고우Mn0.5Zn0.5Fe2O4납미재료연합자류체열료조혹유리 As2O3조(P <0.05).표명 As2O3/Mn0.5Zn0.5Fe2O4복합납미립연합자류체열료가현저억제식관암세포증식.
@@@@BACKGROUND: Compared with traditional hyperthermia treatment, As2O3/Mn0.5Zn0.5Fe2O4 complex may have both the cytotoxicity effects and the targeted heating effects resulting from the radiofrequency-induced hyperthermia. OBJECTIVE: To prepare nanosized As2O3/Mn0.5Zn0.5Fe2O4 complex and to observe its growth inhibition effects on esophageal cancer cells Eca109. METHODS: The As2O3/Mn0.5Zn0.5Fe2O4 complex was made using impregnation process. Its properties were described through transmission electron microscope, energy spectrometer, and atomic fluorescence spectrometer. In in vitro test, two plates of Eca109 cel lines were co-cultured with nanosized As2O3/Mn0.5Zn0.5Fe2O4, Mn0.5Zn0.5Fe2O4 or aqueous of As2O3, and then one of the plates was placed in an alternating magnetic field to rise to a therapeutic temperature, which is cal ed magnetic fluid hyperthermia. The other plate of cells was cultured as usual. RESULTS AND CONCLUSION: The nanosized As2O3/Mn0.5Zn0.5Fe2O4 were approximately global examined by transmission electron microscope. The As content measured by atomic fluorescence spectrophotometer was 0.012% to 0.066%. The proliferation ratio of the cells exposed to As2O3/Mn0.5Zn0.5Fe2O4 complex at the concentration of 5 μmol/L was significantly lower than that exposed to Mn0.5Zn0.5Fe2O4 alone or the negative control group (P < 0.05). The apoptosis rate of cells exposed to As2O3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia was much higher than that exposed alone to Mn0.5Zn0.5Fe2O4 with magnetic fluid hyperthermia or As2O3 (P < 0.05). These findings indicate that As2O3/Mn0.5Zn0.5Fe2O4 complex combined with magnetic fluid hyperthermia can significantly inhibit the proliferation of esophageal cancer cells.