CAJ | 학술논문

背景:前期实验采用溶胶-凝胶法在纯钛片表面制备了纳米 TiO2:C 薄膜涂层.目的:评估纳米 TiO2:C 薄膜涂层的生物相容性.方法:①溶血实验:取新西兰大白兔静脉血,分别加到 TiO2-生理盐水、TiO2:C-生理盐水、蒸馏水和生理盐水中.②细胞毒性实验:将 TiO2:C 浸提液加入对数生长期的 L929细胞培养基中.③短期全身毒性实验:将60只 BALB/C 小白鼠随机分为3组,分别灌胃给予 TiO2浸提液、TiO2:C 浸提液及生理盐水.④口腔黏膜刺激实验:将牙胶圆片与 TiO2:C 纳米薄膜涂片分别缝合固定于仓地鼠颊黏膜上.结果与结论:纳米 TiO2:C 涂层未引起急性溶血,毒性等级为0-1级,未对细胞产生明显毒性,细胞周期未见明显异常;TiO2:C 浸提液对小鼠无短期全身毒性;对仓鼠口腔黏膜无刺激性.表明纳米 TiO2:C薄膜涂层具有良好的生物安全性.
배경:전기실험채용용효-응효법재순태편표면제비료납미 TiO2:C 박막도층.목적:평고납미 TiO2:C 박막도층적생물상용성.방법:①용혈실험:취신서란대백토정맥혈,분별가도 TiO2-생리염수、TiO2:C-생리염수、증류수화생리염수중.②세포독성실험:장 TiO2:C 침제액가입대수생장기적 L929세포배양기중.③단기전신독성실험:장60지 BALB/C 소백서수궤분위3조,분별관위급여 TiO2침제액、TiO2:C 침제액급생리염수.④구강점막자격실험:장아효원편여 TiO2:C 납미박막도편분별봉합고정우창지서협점막상.결과여결론:납미 TiO2:C 도층미인기급성용혈,독성등급위0-1급,미대세포산생명현독성,세포주기미견명현이상;TiO2:C 침제액대소서무단기전신독성;대창서구강점막무자격성.표명납미 TiO2:C박막도층구유량호적생물안전성.
@@@@BACKGROUND: In previous experiments, TiO2:C thin film coating have been prepared on the surface of titanium paper by sol-gel process. OBJECTIVE: To evaluate the biocompatibility of nanometer TiO2:C thin film coating. METHODS: Hemolysis test: New Zealand white rabbit venous blood was extracted and added into the TiO2-normal saline, TiO2:C-normal saline, distil ed water and fresh normal saline. Cytotoxicity test: TiO2:C leaching liquor was added into the L929 cel culture medium in the logarithmic growth phase. Short-term systemic toxicity test: 60 BALB/C mice were randomly divided into three groups and lavaged with leaching liquor extracted from TiO2, TiO2:C coatings and normal saline. Oral mucosa stimulation test: the gutta-percha wafer and the nano-TiO2:C thin film coating were sutured and fixed to the cheek pouch of inaurate hamster. RESULTS AND CONCLUSION: Acute hemolysis was not induced by nano-TiO2:C thin film coating. Toxicity level of nano-TiO2:C thin film coating was 0-1. It had no obvious toxicity to the cells. There was no obvious abnormality of cel cycle. TiO2:C leaching liquor had no short-term systemic toxicity on mice and no stimulation to oral mucosa of hamster. The results showed that nano-TiO2:C thin film coatings have good bio-security.