CAJ | 학술논문

背景:富血小板血浆是目前已知富含多种生长因子并能将其释放的自体提取物,并已应用于骨、软骨组织工程再生的研究.目的:通过比较不同方法制备兔富血小板血浆中血小板浓度,并测定其血小板源性生长因子、转化生因子β1水平,探讨富血小板血浆制备方法及影响因素.方法:采用 Petrungaro 法、Landesberg 法、Aghaloo 法制备新西兰大耳白兔富血小板血浆.检测3组富血小板血浆中血小板计数,以及3组富血小板血浆活化前后及正常血浆、贫血小板血浆中血小板源性生长因子、转化生因子β1水平.结果与结论:3种方法制备的富血小板血浆中血小板计数、血小板回收率、血小板富集系数差异有非常显著性意义(P <0.001),Landesberg 法和 Aghaloo 法均可制备有效浓度的富血小板血浆,且 Aghaloo法制备血小板浓度及活性高于 Landesberg 法(P <0.05).活化前3组富血小板血浆中血小板源性生长因子、转化生因子β1水平与正常血浆组、贫血小板血浆组比较差异无显著性意义.活化后,Landesberg法和 Aghaloo 法制备的血小板血浆中血小板源性生长因子、转化生因子β1水平明显高于活化前(P <0.001),且 Aghaloo 法最高(P <0.05).富血小板血浆中血小板计数与血小板源性生长因子水平(r=0.872, P <0.001),转化生因子β1水平(r=0.917,P <0.001)呈正相关.
배경:부혈소판혈장시목전이지부함다충생장인자병능장기석방적자체제취물,병이응용우골、연골조직공정재생적연구.목적:통과비교불동방법제비토부혈소판혈장중혈소판농도,병측정기혈소판원성생장인자、전화생인자β1수평,탐토부혈소판혈장제비방법급영향인소.방법:채용 Petrungaro 법、Landesberg 법、Aghaloo 법제비신서란대이백토부혈소판혈장.검측3조부혈소판혈장중혈소판계수,이급3조부혈소판혈장활화전후급정상혈장、빈혈소판혈장중혈소판원성생장인자、전화생인자β1수평.결과여결론:3충방법제비적부혈소판혈장중혈소판계수、혈소판회수솔、혈소판부집계수차이유비상현저성의의(P <0.001),Landesberg 법화 Aghaloo 법균가제비유효농도적부혈소판혈장,차 Aghaloo법제비혈소판농도급활성고우 Landesberg 법(P <0.05).활화전3조부혈소판혈장중혈소판원성생장인자、전화생인자β1수평여정상혈장조、빈혈소판혈장조비교차이무현저성의의.활화후,Landesberg법화 Aghaloo 법제비적혈소판혈장중혈소판원성생장인자、전화생인자β1수평명현고우활화전(P <0.001),차 Aghaloo 법최고(P <0.05).부혈소판혈장중혈소판계수여혈소판원성생장인자수평(r=0.872, P <0.001),전화생인자β1수평(r=0.917,P <0.001)정정상관.
BACKGROUND: Platelet-rich plasma is an autogeneic extract containing and releasing a variety of growth factors, which has been used in the research of bone and cartilage tissue engineering and regeneration. OBJECTIVE: To investigate the preparation methods of platelet-rich plasma and its influencing factors by comparing the platelet concentration in the platelet-rich plasma prepared by different methods in rabbits and detecting the levels of platelet-derived growth factor and transforming growth factor β1. METHODS: Platelet-rich plasma was prepared by the methods of Petrungaro, Landesberg and Aghaloo. Platelet amount was counted in the platelet-rich plasma prepared using three methods as wel as platelet-derived growth factor and transforming growth factor β1 levels. RESULTS AND CONCLUSION: There were significant differences in the platelet count, platelet recovery rate and platelet enrichment coefficient in the platelet-rich plasma prepared by these three methods (P < 0.001). The effective concentration of platelet-rich plasma could be prepared by the methods of Landesberg and Aghaloo, and the platelet concentration and activity prepared by Aghaloo method was higher than that prepared by Landesberg method with a statistical difference (P < 0.05). The levels of platelet-derived growth factor and transforming growth factor β1 in the inactivated platelet-rich plasma were not statistical y different from those in the normal plasma and platelet-poor plasma. The levels of platelet-derived growth factor and transforming growth factor β1 in the activated platelet-rich plasma prepared by Landesberg and Aghaloo methods were significantly higher than before activation (P < 0.001), especial y in the activated platelet-rich plasma prepared by Aghaloo method (P <0.05). The platelet count in the platelet-rich plasma was positively correlated with the levels of platelet-derived growth factor (r=0.872, P < 0.001) and transforming growth factor β1 (r=0.917, P < 0.001).